Average customer rating:
- All About Snobbery
- Posture your Carpe Diem, Fools
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A Posturing Of Fools
Brewster Milton Robertson
Manufacturer: River City Pub.
ProductGroup: Book
Binding: Hardcover
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ASIN: 1579660517 |
Book Description
Combining the wistfulness of F. Scott Fitzgerald's "Winter Dreams" with the social criticism of Sloan Wilson's The Man in the Gray Flannel Suit, Brewster Milton Robertson disdains convention with his new novel. Wryly introspective yet bawdy and delightfully erotic and set against the backdrop of nonpareil elegance at the renowned Greenbrier resort A Posturing of Fools introduces hopelessly corruptible Heneley Logan Baird, a testosterone-fueled postmodern Tom Jones, and recounts the crucible experiences of a disillusioned young husband forced to ask disquieting questions about the important values in his life.
Customer Reviews:
All About Snobbery.......2006-11-08
This novel would be enjoyed most by men who enjoy sex and golf, but wives need to read it to avoid being easily duped by straying husbands. On a deeper level, it serves as a social commentary on snobbery and the rat race in which many modern workers find themselves trapped, especially those in sales. A worthwhile and hilarious read.
Posture your Carpe Diem, Fools.......2004-11-11
A Posturing of Fools is such a great read by Brewster Milton Robertson that it inspired me to write a review. Quick, and full of subtle and sometimes laugh-out-loud humor, the story has us watching as H. Logan Baird, a salesman for a pharmaceutical company, tries to juggle his whining wife, a blatantly arrogant and ungrateful boss, his job, the loss of a good friend, and his desire to be with other women, all during a four-day medical conference at the famous Greenbrier Resort in West Virginia.
What I found irresistible about Logan in his trials and tribulations, where he faces the eternal conflict of one who is trying to have his cake and eat it too, is how important his own father's gentlemanly manners creep into and influence the way he treats others (albeit not much for the sanctity of his marriage, mind you). Yes, Logan does have faults and wayward ways that include his overactive testosterone, all of which the reader is lucky enough to be privy to. But his redeeming quality comes from his desire to be a "hands-on-father," choosing to raise his son not from a distance, even if life at home isn't all that great.
What I liked best about A Posturing of Fools is Robertson's masterful skill of the English language: It becomes a banquet of seldom-repeated words and phrases, which enticed me to devour both the story and the feast of its component words.
With the many characters, plots, twists and turns that this 450 + page novel presents, it's surprisingly quite easy to follow, even when the story line enters an area that is foreign to me: golf. But, as Logan often says, "Carpe Diem" (Seize the day).
You'll love the characters...you'll definitely relate to a few...and you may even learn a thing or two about yourself. Enjoy.
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A Posturing of Fools.(Book Review): An article from: Reviewer's Bookwatch
Tracey Broussard
Manufacturer: Midwest Book Review
ProductGroup: Book
Binding: Digital
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ASIN: B0007UUXKA
Release Date: 2005-07-13 |
Book Description
This digital document is an article from Reviewer's Bookwatch, published by Midwest Book Review on January 1, 2005. The length of the article is 535 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.
Citation Details
Title: A Posturing of Fools.(Book Review)
Author: Tracey Broussard
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Reviewer's Bookwatch (Newsletter)
Date: January 1, 2005
Publisher: Midwest Book Review
Page: NA
Article Type: Book Review
Distributed by Thomson Gale
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- Dragonseye
- My favorite
- Great if you like Sci Fiction
- Dragonseye
- Dragonseye
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Dragonseye
Anne McCaffrey
Manufacturer: Del Rey
ProductGroup: Book
Binding: Mass Market Paperback
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Dragonsdawn (Dragonriders of Pern Series)
ASIN: 0345418794
Release Date: 1997-12-27 |
Amazon.com
When the volcanoes rumble and the powerful storms begin brewing on Pern, it means one thing: Thread. For 257 years Pern has been free of the life-destroying Thread, but now the Red Star has reappeared in the sky and soon the deadly Threadfall will follow. In the holds and weyrs across the land, the genetically-engineered dragons of Pern and their human riders begin feverishly training to combat the Thread, for only dragon fire can destroy the silvery invaders. But, incredibly, one Lord Holder refuses to believe the Thread will fall again, and he may endanger the entire planet.
Book Description
In Anne McCaffrey's New York Times bestselling DRAGONSEYE,
join Weyrleaders, Holders, and Craftmasters in the creation of the legendary Star Stones and the teaching ballads of Pern!
It's been two-hundred years since the deadly Thread fell like rain upon Pern, devouring everything in its path. No one alive remembers that first horrific onslaught and no one believes in its return--except for the dragonriders. For two centuries they have been practicing and training, passing down from generation to generation the formidable Threadfighting techniques.
Now the ominous signs are appearing: the violent winter storms and volcanic eruptions that are said to herald the approach of the Red Star and its lethal spawn. But one stubborn Lord Holder, Chalkin of Bitra, refuses to believe--and that disbelief could spell disaster. So as the dragonriders desperately train to face a terrifying enemy, they and the other Lord Holders must find a way to deal with Chalkin--before history repeats itself and unleashes its virulence on all of Pern. . . .
Download Description
It's been two hundred years since the deadly Thread fell like rain uponPern, devouring everything in its path. No one alive remembers thatfirst horrific onslaught and no one believes in its return -- except forthe dragonriders. For two centuries they have been practicing andtraining, passing down from generation to generation the formidableThreadfighting techniques.
Now the ominous signs are appearing: the violent winter storms andvolcanic eruptions that are said to herald the approach of the Red Starand its lethal spawn. But one stubborn Lord Holder, Chalkin of Bitra,refuses to believe -- and that disbelief could spell disaster. So as thedragonriders desperately train to face a terrifying enemy, they and theother Lord Holders must find a way to deal with Chalkin -- beforehistory repeats itself and unleashes its virulence on all of Pern...
"[McCaffrey is] a master of the well-told tale."
CHICAGO SUN-TIMES
"If you are a fan of Anne McCaffrey's Dragons of Pern series, you willlove Dragonseye."
ANALOG
"Science fiction's most famous dragons are back in another thrillingchapter in the saga of the world of Pern... McCaffrey's spellbindingdragons win our hearts and minds in another glorious adventure."
ROMANTIC TIMES
"McCaffrey brings us another diverse cast of responsible, heroic goodguys and dragons in a novel that's going to please fans old and new --and probably burn up the charts like dragon's fire."
PUBLISHERS WEEKLY
"Meticulously detailed... Should satisfy most dragon fans."
KIRKUS REVIEWS
Cover art by Eric Peterson
Customer Reviews:
Dragonseye.......2007-10-02
Enjoyed the story; Wondered in previous books about how the dragon riders knew when thread was due. This story answered my question.
My favorite.......2007-07-11
Probably my favorite Pern book although it is in compitition with The Skies of Pern. However I would recomend reading the original Dragonriders of Pern trilogy first, it will give you a better understanding of the other Pern novels. I hope to see another book about Iantine and Debera. This book seemed to be written with at least one sequel in mind, but none have been published. I admit to being homophobic so it is understandable that I prefer female green riders. But considering the link between dragon and rider I wondered in the first Dragonrider novels why green(female) dragons were impressed by men when it would make much better since for them to be impressed by women.
Great if you like Sci Fiction.......2007-05-13
The Pern series of books from Anne McCaffery are great. Better if you read them in order, (look online at her website for recommendations). This is futuristic science fiction, but if you liked Aregon, you would probably like this series too
Dragonseye.......2007-01-22
Another winner!!!!! I love finally knowing how and why Pern has developed the way it has!
Dragonseye.......2005-09-18
As before, this is a series I started and never finished years ago that I have the opportunity to reread and complete now. It is very enjoyable to escape to the world that Anne Mccaffrey has created.
Book Description
Dragonseye:
Thread: deadly silver strands that fall from the sky like rain, devouring every organic thing in their path - animals, plants, and people alike. Who could believe that such a horrible thing could exist? After all, it's been two hundred years since Thread supposedly fell on Pern. No one alive remembers that first onslaught. There's no sign of it anywhere in the world. Only the dragons, originally created to be a weapon against Thread, are still around to remind people that once before their population was decimated, their hopes and dreams and livelihoods almost destroyed forever.
For two centuries the dragonriders have been practicing and training, passing down from generation to generation the Threadfighting techniques learned on the fly by their besieged ancestors. And most of the Lord Holders are prepared to protect their people, to provide sanctuary, to assemble groundcrews to search out and destroy any Thread that might be missed by the dragons soaring overhead. All but one.
Even now the ominous signs are appearing: the violent winter storms and volcanic eruptions that are said to herald the approach of the Red Star and its lethal spawn. Impossibly, one stubborn Lord Holder, Chalkin of Bitra, refuses to believe - and that disbelief could spell disaster for all of Pern. So while the dragonriders desperately train to face an enemy they've never fought before, they and the other Lord Holders must find a way to deal with Chalkin and protect Bitra.
The Masterharper of Pern:
In a time when no Thread has fallen for centuries - when, indeed, many are beginning to dare to hope that Thread will never fall again - a boy is born to Harper Hall. His name is Robinton, and he is destined to be one of the most famous and beloved leaders Pern has ever known.
It is a perilous time for harpers. They sing of Thread, yet more and more people are beginning to doubt the return of that deadly scourge. They teach reading, writing, history, but Fax - who hates the harpers in general - is determined to keep his growing area of influence free of the learning that might sow unrest. And they extol the dragonriders, whom many view increasingly as a drain on the resources of the Holds. Now harpers are being turned away from the holds; and, worse yet, they are being derided, attacked, even beaten.
It is the climate of unrest that Robinton will come into his own. For despite the tragedies that beset his own life, he continues to believe in music and in the dragons, and is determined to save his beloved Pern from itself . . .so that the dragonriders can be ready to fly against the dreaded Thread when it at last returns!
The Skies of Pern:
Now that Pern can look forward to a future without the threat of Threadfall, the people are free to leave their protective stone holds and spread across more of the planet, as well as improve their lives with the newly discovered ancient technology. Not everyone is happy, though. Some resist the change, and consider anything new to be an "abomination." And the dragonriders are uncertain: without Thread, what will their purpose be in Pernese society?
Then a new danger - again from the skies - looms. Once again, the people must pull together . . . And turn to the only ones who can solve the crisis: the dragonriders of Pern!
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Dragonseye
Anne McCaffrey
Manufacturer: Del Rey
ProductGroup: Book
Binding: Paperback
ASIN: B000OVOS80 |
Average customer rating:
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Dragonseye
Manufacturer: Turtleback Books Distributed by Demco Media
ProductGroup: Book
Binding: Hardcover
ASIN: 060628382X |
Average customer rating:
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Dragonseye
Anne McCaffrey
Manufacturer: Del Rey
ProductGroup: Book
Binding: Paperback
ASIN: B000OCO3B6 |
Average customer rating:
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Dragonseye
Anne McCaffrey
Manufacturer: Brilliance Corporation
ProductGroup: Book
Binding: Audio Cassette
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ASIN: B000O3FSJG |
Product Description
Dragonriders of Pern novel
Average customer rating:
- Read It In Two Days - Wondering Why I Bothered.
- How does Sawyer cram so much into one book?
- Badly Handled Story.
- MIND-EXPANDING IDEAS - GREAT STORY
- I only read it to the end for the comedy value
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Frameshift
Robert J. Sawyer
Manufacturer: Tor Books
ProductGroup: Book
Binding: Paperback
Sawyer, Robert J.
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ASIN: 0765313162
Release Date: 2005-10-13 |
Amazon.com
There is a 50 percent chance that geneticist Pierre Tardivel is carrying the gene for Huntington's Disease, a fatal disorder. That knowledge drives Pierre in his work on the Human Genome Project, an attempt by scientists to map human genes. But a strange set of circumstances--including a knife attack, the in vitro fertilization of his wife, and an insurance company plot to use DNA samples to weed out clients predisposed to early deaths--draw Tardivel into a story that will ultimately involve the hunt for a Nazi death camp doctor. Frameshift shows why the New York Times calls Robert J. Sawyer "a writer of boundless confidence."
Book Description
This is the story of Pierre Tardivel, a scientist, and his complex battle against deadly illness; an ex-Nazi war criminal still hiding in the US; a crooked insurance company; and a plot to make Pierre and his wife the victims of a bizarre genetic experiment. Sawyer juggles his plots smoothly and gracefully, and never drops a ball. Frameshift is hard science fiction at its best, full of complications and neat surprises.
Customer Reviews:
Read It In Two Days - Wondering Why I Bothered........2006-11-16
I was excited to read Frameshift at first. A small-scale adventure, confined to one planet, to one species - humans. I didn't see how Sawyer could pull his usual trick of cramming too much subject matter into a book for its own good here, and thankfully, Sawyer manages to focus pretty well on his subject - an unusual achievement for him.
Unfortunately, though, the book suffers from a flawed structure, and the conclusion is undramatic and abrupt. I don't like it when an author has trouble exploiting the dramatic potential latent in his concepts, and Sawyer might as well just not write at all if he can't cure this problem.
If a Nazi is on the loose, shouldn't most of the book center around him? It sort of does, better than most of Sawyer's work, but I was irritated by the insurance company angle taking up so much time in the book, and the science seemed irrelevant and pointless.
By the end of the novel, I still didn't understand what any of the science meant, and I was worried this would stop me from understanding the climax. But this didn't turn out to be a problem, since the villain just gets blown up. This opens the question of why Sawyer bothered wasting time including the science at all. Things that don't drive the plot of a novel or contribute in some signifigant way should be left out.
Robert J. Sawyer seems to have trouble just picking one extraordinary thing and sticking to it. Most of his books are irritating, having such a mish-mash of different subjects all piled together that it's hard to know which one to concentrate on. Frameshift is one of his best, but it still amounts up to a dull, unfullfilling conclusion.
How does Sawyer cram so much into one book?.......2006-11-15
This is the second novel I have read by Canadian author Robert J. Sawyer and after this I will definitely be reading a lot more. I am in amazement about some of the reviews listed here. It appears that if an author has a comment or idea that makes some of these reviewers uncomfortable or the plot does not go the way they want it to then the author must be at fault. Now personally I enjoyed being challenged by a writer to keep my mind open and let new thoughts slip in, and in Sawyer's books the ideas are exploding like hot popcorn kernels.
This novel runs the gamut of ideas, plots and sub-plots. Sawyer covers everything from Huntington's disease, telepathy, evil nazis, neanderthals, crooked insurance companies, and genetics in less than 350 pages and makes it all work smoothly. With a strong narrative style, believable characters, and an ability to explain complex scientific principles in a manner that is easy to comprehend Sawyer keeps the reader turning the pages. Several of the reviewers have criticized this book saying that having an evil or corrupt insurance company as the villain is too unrealistic. If I am not mistaken insurance companies are interested in one thing and one thing only-profit. If they could find a way (and in this book they do) to increase the odds in their favor, they would.
If you want a science-fiction book that actually has science in it, some controversial and original viewpoints and is bursting at the seems with ideas and is well-written as well, then you should enjoy Robert J. Sawyer's FRAMESHIFT.
Badly Handled Story........2006-11-07
I bought Frameshift because of the Nazi war criminal angle. I always enjoy a good Nazi courtroom prosecution scene, and I thought this book was going to be another one of those. Sadly, it was not. Robert J. Sawyer even mentions one of my favorite movies in the novel, Judgment at Nuremburg, which may very well be the ultimate Nazi trial movie; but the novel completely fails to live up to the greatness of that incredible film.
I didn't get any of the drama or courtroom intrigue I was expecting in Frameshift. The book reads quickly enough, and the writing is pleasant, but the payoff at the end is a colossal letdown. I love watching defiant Nazis led to the stand, unrepentant and demonic, demanding their freedom and resisting all efforts to establish their guilt. Robert J. Sawyer gives us none of that, and I felt cheated by the quick death of the villain. (Oops.)
"Ivan the Terrible," as he is called, may not always be visible throughout the book, but his presence drives the action in almost every scene. His existence and his vile deeds torment the good guys, and I wanted to see him suffer. After a whole book of him making ME suffer, why not? I wanted to see him go to trial. I wanted to see him confronted with his crimes and the people he hurt. But that never happens.
The survivors of the Nazi death camp Treblinka are eventually called to testify against their tormentor, but this material appears to be tacked onto the book for no apparent reason, since the bad guy never appears in court. And the entire reference to Judgement at Nuremburg, though skillfully handled, gives completely the wrong courtroom vibe to a book in which a courtroom utterly fails to materialize.
Why, Robert J.? Why did you cheat us? You set the scene beautifully but failed to carry through.
I have noticed that a lot of Robert J. Sawyer's books seem to have structural and dramatic problems in them. I tried to read the first book in his Neanderthal Parallax series, but I gave up when I realized it was dreadfully paced, the characters being handled in abrupt, heavy-handed ways. These character problems are not evident in Framshift, but this book is also very badly structured, promising untold courtroom delights, but conking out at the crucial moment.
None of Sawyer's books have ever been turned into big sucessful movies the way Micheal Crichton's books have, and I can see exactly why. Sawyer has zero cinematic sense. The action is practically non-existant, and we are presented with oodles and oodles of thinking and character work, not always very good. And when the action finally does come, it is the wrong kind. This book promised to be a tensely plotted courtroom thriller. All the elements were there. And none of it came to pass.
The science, in Sawyer's books, is often quite dull and unoriginal, and it gets on my nerves. Micheal Crichton, at least, understands how to weave some beautifully original research into a finely balanced action-adventure storyline, but Sawyer's science seems boring and pedestrian by comparison. It just isn't exciting. I could easily have skipped most of the genetic science bits in the story without losing any of the actual plot (which is, in fact, what I did.) Shame on you, Robert. Shame.
To be fair, though, Sawyer does have some talent. It's just that he keeps using his talent to create distasteful, unfullfilling novels. He keeps his characters fairly consistent (at least in this book) and he sticks to his theme without too many deviations. But what he does with his story just doesn't work, and it is disappointing on almost every level.
Looking back, I can see that Sawyer was carefully setting up his plot so that the bad guy (disappointingly) gets blown up at the end. All the steps were there, but I just couldn't see them until I'd actually finished the book. All the villain's wicked deeds are gradually unmasked as the story progresses, and so naturally enough, a highly-charged trial doesn't make any sense by the end. There is really nothing new to do with the character, and Sawyer really has no choice but to blow him up. A perfectly logical, perfectly rational way of creating a really pointless story. A total failure.
MIND-EXPANDING IDEAS - GREAT STORY.......2006-09-03
I thoroughly enjoyed this book which examines some important moral and scientific issues. I liked Pierre and Molly and found the information on DNA fascinating. But I think the author went off in too many directions, with the Nazi stuff (incorporating true events involving the false accusations against John Demjanjuk), the crazy old guy studying Neanderthal DNA, the findings on DNA frameshifting, and the greedy insurance company with its murderous founder and rapacious stockholders.
Unlike some of the other reviewers here, I found Sawyer's implied criticism of the US health care system to be fully justified, but this Canadian author did not get it all right. In some ways, the situation in the US is even worse than he portrayed. Someone like Pierre, trying to get health insurance on his own, would find that even if he was perfectly healthy all he could get was very poor coverage at very high rates. The only people in the US who have great coverage for health care expenses are people who work for large companies and have employer-provided insurance or are public employees. But Pierre would probably not have been left to get his own insurance because, as Molly's husband, he could probably have been added to her insurance and, since she was a public employee, she would have had very good coverage. The profit-driven US health care system (not really a "system" at all - more like a lottery) arbitrarily provides great health care to a lucky group, mediocre care to most, and no care at all to millions of unlucky Americans.
Surely someone in Pierre's situation - having Huntington's Disease - would go back to Canada where he would never have to worry about getting the care he needed or going broke trying to pay for it. Yes, I totally envy the Canadians for their fair and humane health care system. In the US, we have created a "health care system" that tries to avoid anyone who actually needs health care! (and could theoretically lead to schemes to eliminate people whose health care will be very expensive).
Okay, some of the plot elements were a little far out, but the drama moved along nicely and I identified with Molly's desire to be a mother and found myself really wondering what I would do in her situation (not to give away the plot here, but I mean what she found out about her in-vitro conceived daughter). When I got to that part of the book, I almost had to stop reading because for me the sheer horror of what was done was just too much. But Molly so loves her little girl that nothing matters, and her gift of reading thoughts turns out to have a purpose. A bit cornball, but still touching. I give the book high marks for making readers think, even those who disagreed with the author on some issues.
I only read it to the end for the comedy value.......2006-08-30
I'd call this book unintentionally hilarious. The science parts are not as awful as in other books of the genre, but the writing is wooden, the characters are flat and the abrupt insertion of long quotes from other books and works of political oratory is annoying. But that's not why I call it unintentionally hilarious. No, that's because the bad guy is ... (drumroll) an evil insurance company! An evil insurance company that engages in a secret plot so that it can keep its actuarial tables up to date, no less! Plus you just can't beat lines like this: "What better work for an out-of-work Nazi than being an actuary?"
Book Description
This digital document is a journal article from Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Abstract:
To facilitate detection of genotoxicity from environmental mutagen exposure, we generated an in vitro enhanced green fluorescence protein (EGFP) reactivation assay that quickly and effectively detects frameshift mutations in tandem repeat sequences (TRS). Two murine cell lines, C3H10T1/2 and mismatch repair deficient MC2a, were stably transfected with EGFP reporter plasmids in which the EGFP constructs contain TRS that put the EGFP sequence out of frame. These included several 2, 3, 4, 5 and 6bp repeat sequences, a control non-repetitive sequence and a human gene sequence containing a 4bp repeat motif. Transfected cultures were exposed to five model mutagens and carcinogens: hydrogen peroxide (H"2O"2), 12-O-tetradecanoyl-phorbol-13-acetate (TPA), benzo-a-pyrene-diol-epoxide (BPDE), ethyl nitrosourea (ENU), 9-aminoacridine (9AA) and two controls: acetone and ethanol. Frameshift mutations resulted in green fluorescent revertants, as determined by flow cytometry, and were confirmed, for 9AA treatments, by sequencing. All five treatments with model agents induced statistically significant sequence- and exposure-dependent responses in MC2a cells and a negative response with the two negative control treatments, acetone and ethanol. Similar responses were seen in a smaller panel of treatments and plasmids in C3H10T1/2 cells. The mutation frequencies were higher in cells transfected with the plasmids containing TRS than those harbouring the control construct lacking repeats. The highest mutation frequencies were observed with H"2O"2 and 9AA treatments, yielding up to a 50-fold difference between vehicle and highest concentration treatment. ENU, BPDE, and to a lesser extent TPA treatments, also showed a statistically significant exposure response. Results from these experiments reveal that the assay responds robustly to various classes of mutagenic substances, as well as to rodent carcinogens that are inactive in conventional mutation assays, and that responses are not linked to cytotoxicity. This assay is a promising approach for detecting chemically induced frameshifts within certain DNA sequences of interest, but further characterization and validation are required prior to general use in genotoxicity screening.
Book Description
This digital document is a journal article from DNA Repair, published by Elsevier in 2007. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Spontaneous frameshift mutations are an important source of genetic variation in all species and cause a large number of genetic disorders in humans. To enhance our understanding of the molecular mechanisms of frameshift mutagenesis, 583 spontaneous Trp^+ revertants of two trpA frameshift alleles in Escherichia coli were isolated and DNA sequenced. In order to measure the contribution of methyl-directed mismatch repair to frameshift production, mutational spectra were constructed for both mismatch repair-proficient and repair-defective strains. The molecular origins of practically all of the frameshifts analyzed could be explained by one of six simple models based upon misalignment of the template or nascent DNA strands with or without misincoroporation of primer nucleotides during DNA replication. Most frameshifts occurred within mononucleotide runs as has been shown often in previous studies but the location of the 76 frameshift sites was usually outside of runs. Mismatch repair generally was most effective in preventing the occurrence of frameshifts within runs but there was much variation from site to site. Most frameshift sites outside of runs appear to be refractory to mismatch repair although the small number of occurrences at most of these sites make firm conclusions impossible. There was a dense pattern of reversion sites within the trpA DNA region where reversion events could occur, suggesting that, in general, most DNA sequences are capable of undergoing spontaneous mutational events during replication that can lead to small deletions and insertions. Many of these errors are likely to occur at low frequencies and be tolerated as events too costly to prevent or repair. These studies also revealed an unpredicted flexibility in the primary amino acid sequence of the trpA product, the @a subunit of tryptophan synthase.
Book Description
This digital document is a journal article from DNA Repair, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Progression of DNA replication is occasionally blocked by endogenous and exogenous DNA damage. To circumvent the stalling of DNA replication, cells possess a variety of specialized DNA polymerases that replicate through DNA damage. Salmonella typhimurium strain TA1538 has six DNA polymerases and four of them are encoded by damage-inducible SOS genes, i.e. polB"S"T (pol II), dinB"S"T (pol IV), umuDC"S"T (pol V) and samAB. The strain has been used for the detection of a variety of chemical mutagens because of the high sensitivity to -2 frameshift occurring in CGCGCGCG sequence. To assign the role of each DNA polymerase in the frameshift mutagenesis, we have constructed the derivatives lacking one or all of SOS-inducible DNA polymerases and examined the mutability to 26 chemical mutagens. Interestingly, the chemicals could be categorized into four classes: class I whose mutagenicity was reduced by the deletion of dinB"S"T (1-aminoanthracene and other four chemicals); class II whose mutagenicity was reduced by the deletion of either dinB"S"T or umuDC"S"T plus samAB (7,12-dimethylbenz[a]anthracene and other three chemicals); class III whose mutagenicity largely depended on the presence of umuDC"S"T plus samAB (1-N-6-azabenzo[a]pyrene and other three chemicals) and class IV whose mutagenicity was not reduced by deletion of any of the genes encoding SOS-inducible DNA polymerases (Glu-P-1 and other 12 chemicals). Deletion of polB"S"T reduced by 30-60% the mutagenicity of six chemicals of classes II and III. These results suggest that multiple DNA polymerases including the replicative DNA polymerase, i.e. DNA polymerase III holoenzyme, play important roles in chemically induced -2 frameshift and also that different sets of DNA polymerases are engaged in the translesion bypass of different DNA lesions.
Book Description
This digital document is a journal article from DNA Repair, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
DNA replication is frequently hindered because of the presence of DNA lesions induced by endogenous and exogenous genotoxic agents. To circumvent the replication block, cells are endowed with multiple specialized DNA polymerases that can bypass a variety of DNA damage. To better understand the specificity of specialized DNA polymerases to bypass lesions, we have constructed a set of derivatives of Salmonella typhimurium TA1538 harboring plasmids carrying the polB, dinB or mucAB genes encoding Escherichia coli DNA polymerase II, DNA polymerase IV or DNA polymerase RI, respectively, and examined the mutability to 30 chemicals. The parent strain TA1538 possesses CGCGCGCG hotspot sequence for -2 frameshift. Interestingly, the chemicals could be classified into four groups based on the mutagenicity to the derivatives: group I whose mutagenicity was highest in strain YG5161 harboring plasmid carrying dinB; group II whose mutagenicity was almost equally high in strain YG5161 and strain TA98 harboring plasmid carrying mucAB; group III whose mutagenicity was highest in strain TA98; group IV whose mutagenicity was not affected by the introduction of any of the plasmids. Introduction of plasmid carrying polB did not enhance the mutagenicity except for benz[a]anthracene. We also introduced a plasmid carrying polA encoding E. coli DNA polymerase I to strain TA1538. Strikingly, the introduction of the plasmid reduced the mutagenicity of chemicals belonging to groups I, II and III, but not the chemicals of group IV, to the levels observed in the derivative whose SOS-inducible DNA polymerases were all deleted. These results suggest that (i) DNA polymerase IV and DNA polymerase RI possess distinct but partly overlapping specificity to bypass lesions leading to -2 frameshift, (ii) the replicative DNA polymerase, i.e., DNA polymerase III, participates in the mutagenesis and (iii) the enhanced expression of E. coli polA may suppress the access of Y-family DNA polymerases to the replication complex.
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