Immunoassays: A Practical Approach (Practical Approach Series)

Book Description

Immunoassays is the first practical volume designed to help any biologist develop an immunoassay of any common format for any suitable analyte. The basic principles are described and the choices of assay types and formats listed. Methods for raising and for characterizing antibodies, for preparing radioactive, enzymatic, fluorescent and other labels, are described. Solid-phase reagents, standards and immunogens are explained, with procedures for their preparation. But most components can be obtained commercially and comprehensive lists of suppliers are given. Approaches to combining the reagents and optimizing the required assay are described, including how to eliminate common interferences, and thorough validation procedures are given. Manual and computer methods for the calculation of concentrations are explained. Since only assays that are within control give valid results, a graded and comprehensive approach to internal and external quality assurance methods is detailed, including how to prepare control samples.

Antibodies: A Practical Approach Volume 1 (Practical Approach Series)

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Antibodies: A Practical Approach Volume 1 (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

Immunology | Basic Science | Medicine | Subjects | Books

Book Description

Antibodies have become the preferred, and often essential, tools to identify, quantify and probe the structure and biological properties of an enormous range of antigenic molecules. This volume describes the properties of antibodies and antigens and provides detailed protocols for the
production and quality control of polyclonal, murine monoclonal and human monoclonal antibodies. Step-by-step descriptions are given for antibody methods. In addition to giving precise methodological details, the contributors consider the applications and appropriate use of these
techniques.

ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects

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ELISA and Other Solid Phase Immunoassays: Theoretical and Practical Aspects

Manufacturer: Wiley
ProductGroup: Book
Binding: Paperback

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ASIN: 0471909823

Book Description

This is a quick-reference manual on practical aspects of immunoassay. Providing a theoretical and practical basis for modern applications of solid-phase immunoassays, this text brings together experts who have used ELISA and other assays in a variety of fields. Contributors offer step-by-step guidance on how to use the various techniques involved in immunoassay. These techniques are extemely useful to laboratory-based researchers and technicians working on the detection of allergy, the AIDS virus, autoimmunity, etc. Chapters analyze the solid-phase supports used, the amplification systems, and the quantitation and affinity of antibodies and discuss the applications of assays to biology, immunology, and microbiology.

Immunoassay Automation: A Practical Guide

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Immunoassay Automation: A Practical Guide

Manufacturer: Academic Pr
ProductGroup: Book
Binding: Paperback

Biochemistry | Biological Sciences | Science | Subjects | Books

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Immunoassay: A Practical Guide
Daniel W. Chan
Manufacturer: Academic Pr
ProductGroup: Book
Binding: Paperback

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ASIN: 0121676358

Immunoassay: A Practical Guide (Ellis Horwood Series in Pharmaceutical Technology)

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Immunoassay: A Practical Guide (Ellis Horwood Series in Pharmaceutical Technology)

Manufacturer: CRC
ProductGroup: Book
Binding: Library Binding

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

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ASIN: 0748405607

Book Description

Immunoassay development is a multidisciplinary activity involving a wide range of skills possessed by few laboratories. This presentation of tried and tested methods should enable scientists and researchers in the pharmaceutical and related industries to more rapidly and effectively develop immunoassays upon which their work is becoming heavily dependent.; Important methods are included for preparing Lapten-protein conjugates and raising the necessary antibodies, concentrating on polyclonal sera, as well as methods for the synthesis of radio and enzyme labelled tracers. Particular attention is paid to the requirements of the regulatory authorities such as the FDA (Food and Drug Administration) with respect to assay validation. Further chapters deal with assay development and optimization, curve fitting and quality control procedures.

Practical Immunoassay (Clinical and Biochemical Analysis)

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Practical Immunoassay (Clinical and Biochemical Analysis)
W. R. Butt
Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

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Quantitative Immunoassay: A Practical Guide for Assay Establishment, Troubleshooting, and Clinical Application

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Quantitative Immunoassay: A Practical Guide for Assay Establishment, Troubleshooting, and Clinical Application
James T. Wu
Manufacturer: AACC Press
ProductGroup: Book
Binding: Paperback

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Immunoassays: A Practical Approach (Practical Approach Series)

ASIN: 1890883336

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Immunoassays: A Practical Approach
James P. Gosling
Manufacturer: Oxford University Press(UK)
ProductGroup: Book
Binding: Paperback
ASIN: B000OKYAYI

Book Description

This book helps readers develop the quantitative literacy skills and savvy needed to function effectively in society and the workplace. It focuses on "mathematical modeling" and the use of elementary mathematics--e.g., numbers and measurement, algebra, geometry, and data exploration--to investigate real-world problems and questions. It assumes no technology other than the use of graphing calculators, and provides a comprehensive technology support system on an accompanying CD-ROM and web site. Linear Functions and Models. Quadratic Functions and Models. Natural Growth Models. Exponential and Trigonometric Models. Polynomial Models and Linear Systems. Optimization Problems. Bounded Growth Models. For anyone wanting to develop proficiency in mathematical modeling.

Book Description

For more than 25 years Marissa Batt has tried cases for the People in every one of the 35 courtrooms at the Criminal Courts Building in downtown Los Angeles, cases ranging from rape and sodomy to armed robbery and murder. Despite her years immersed in violence and its concomitant pain and suffering, Batt has never become hardened or jaded. She has an unshakable belief that justice prevails, whether it happens inside or outside a courtroom. In the pages of this slap-in-the-face look at the criminal justice system (Kirkus Reviews), she presents three of her most difficult and terrifying cases. Each more gripping and moving than the last, they showcase the tremendous courage and humanity of the victims. One case was an eye-popping combination of rape, attempted murder, and arson; another was a brutal same-sex rape; and the third was a vicious vigilante-style murder. Try as you might to look away, you cannot. Told in a voice full of grit as well as compassion, this is true crime at its best.

Customer Reviews:

An Intriguing Picture..........2004-08-25

...of the L.A. justice system. I won't repeat the other reviews, but two or three things deserve note. First, Johnnie Cochran wrote the introduction...high praise indeed. Second, the appendix - "Twenty-five rules for giving effective testimony" - is interesting reading in itself. Overall, the book is not compelling reading, not forcing you to read it in one sitting, but it's interesting and varied. I consumed it in three sittings if memory serves. The pace is generally good, although I felt the Buddhism dragged a bit, but that was only a couple of instances. So - recommended - *especially* if you are a fan of mysteries set in Los Angeles! I await Marissa Batt's next work with more than a little interest.

Awesome True Crime Book.......2004-08-11

What makes this book so compelling is that you get to hear the perspective of the actual prosecutor in the cases described. Through Marissa Batt's words, you get a virtual tour of the way the Los Angeles legal system works, or in some cases doesn't work. You are introduced to characters that actually inhabit Los Angeles, who actually went through the situations described - cases that are so intense and bizarre, they seem unbelievable. Through it all, you get a comprehensive version of the legal system mirroring human lives and decisions, as seen through the eyes of a person who does their job with integrity, intensity, and strength of character. READ THIS BOOK! It is a thorougly enjoyable read though shocking and extremely upsetting at times.

Compelling, compassionate, committed.......2004-03-03

Marissa is erudite, quick, fun, funny, committed to social justice and truth, and and an acute observer of human behavior. Her friend Johnnie Cochran describes her (in the introduction!) as "a prosecutor's prosecutor. She is full of zeal for her profession and possesses an insider's knowledge of the criminal justice system." She's also devoted herself to Buddhism for over 30 years, as well as to the mastery of the culinary arts. All of these elements figure in her very unusual book.

Besides telling three compelling and hair-raising tales, Marissa shares aloud the unspoken rules of the courtroom, and offers appreciative and insightful looks into the lives of law enforcement professionals, and denizens of South Central LA and the gay demimonde of Hollywood.

As a skillful storyteller, she is compassionate without becoming maudlin, and righteous without losing her sense of humor.

I am looking forward to her next book, which I understand is under way!

Justice with a heart.......2004-02-10

Marissa has the heart of a warrior. Her stories are classic story telling with spell binding revelations of what the truth is when it comes to crimes and the criminal mind. Her attention to detail, coupled with a sense of the world of the victim, allows the reader to be a prosecutor seeking justice for the people. The criminal procedural aspects of the criminal courts are cleanly explained with no chance of misunderstanding that lawyering is still an art when done with a heart. Marissa injects the calmness of her buddhist philosophy into the psyche of the reader to allow a deeper appreciation of the law and the victim equation.

Ready for Ms. Batt.......2004-02-06

As a criminal defense attorney, I usually avoid "true accounts" written by prosecutors as they tend to be one-sided, self-serving renditions of cases that could be won by a first year law student. The usual story involves a clear cut case where the District Attorney is representing the forces of right (the good people of the State) while the defense is usually some bad dude who deserves a long vacation in Prison. Of course the good guys prevail and the prosecutor is the hero. Good and Evil, Right and Wrong are clearly defined and everybody leaves happy. Not so with Ms. Batt's book. Besides her personal disappointment at the result of one of her cases, she manages to show that all is not black and white - urban life and particularly the criminal justice system present a myriad of situations where the lines become blurred. Her cases are interesting in that there are victims - individuals who by virtue of their own life choices are often viewed as not deserving of protection by the law and the system. Besides showing the underside of life, Batt also manages to forcefully demonstrate the maxim that "no man is above the law and no man is below it."
A great read, colorful, fast paced and real...I loved it.

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