Transgenic Plants: Methods and Protocols (Methods in Molecular Biology)

Book Description

A collection of readily reproducible techniques for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems for the regeneration of whole transgenic plants. Among the techniques discussed for the detection of transgenes are quantitative real-time PCR, RT-PCR, in situ hybridization, FISH, and TAIL-PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Arabidopsis Protocols (Methods in Molecular Biology)

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Arabidopsis Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588293955

Book Description

This collection of readily reproducible Arabidopsis protocols has been updated to reflect recent advances in plant biology, the completion of the Arabidopsis genome sequence-essential for studying plant function, and the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics. The authors have included nearly all techniques developed in Arabidopsis, others recently adapted from the traditional work in crop species, and the most recent ones using Arabidopsis as a model system. Highlights include the most recent methods-transcriptomics, proteomics, and metabolomics-and their novel applications (phosphoproteomics, DNA microarray-based genotyping, high throughput metabolite profiling, and single-cell RNA).

Plant Cell Electroporation And Electrofusion Protocols (Methods in Molecular Biology)

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Plant Cell Electroporation And Electrofusion Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Plastic Comb

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ASIN: 0896033287

Book Description

Leading experts and innovators describe in detail plant cell electroporation and electrofusion techniques for many types of plants. Their protocols cover a diverse set of plant cell types, including different cell types from a single organism (i.e., leaf cells and pollen) and the most important model plants, including maize and tobacco. Each protocol also contains detailed instructions for growth and growth optimization, protoplast generation, and protoplast regeneration for specific plant cell types, along with extensive troubleshooting advice and descriptions of expected results to simplify study design.

Plant Cell Culture Protocols (Methods in Molecular Biology)

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Plant Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588295478

Book Description

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Arabidopsis: A Laboratory Manual (Spiral)

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Arabidopsis: A Laboratory Manual (Spiral)
Detlef Weigel , and Jane Glazebrook
Manufacturer: Cold Spring Harbor Laboratory Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0879695730

Book Description

The thale cress Arabidopsis thaliana is increasingly popular among plant scientists: it is small, easy to grow, and makes flowers, and the sequence of its small and simple genome was recently completed. This is the most complete and authoritative laboratory manual to be published on this model organism and the first to deal with genomic and proteomic approaches to its biology.

Agrobacterium Protocols: Volume I (Methods in Molecular Biology)

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Agrobacterium Protocols: Volume I (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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Agrobacterium Protocols (Methods in Molecular Biology)

ASIN: 1588295362

Book Description

A gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Arabidopsis: A Practical Approach (Practical Approach Series)

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Arabidopsis: A Practical Approach (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

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ASIN: 0199635641

Book Description

Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.

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Cloning Vectors, A Laboratory Manual
P. H. Pouwels , B. E. Enger-Valk , William J. Brammar , W.J. Brammar , B.E. Enger-Valk , and P.H. Pouwels
Manufacturer: Elsevier Science & Technology
ProductGroup: Book
Binding: Ring-bound

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Book Description

Loose-leaf Binder. 'Cloning Vectors', the very first comprehensive compilation of the presently existing vectors used in cloning, was published in December 1985. As a laboratory manual, it serves in rapidly identifying a given vector best suited for a particular purpose. Because of the extremely rapid developments in the field, annual supplements are published (starting from 1986), which comprise newly identified vectors. The first two supplementary updates are now available.

A large section of the manual is devoted to Escherichia coli, the most commonly used host for general cloning and manipulation of DNA sequences. However, other Gram-negative bacteria are also discussed, as are several Gram-positives, such as Bacillus subtilis, the genera Staphylococcus, Streptococcus and Streptomyces, the lower eukaryotic yeasts and fungi, and animal and plant cells in culture.

Book Description

First published in 1988 and now known as a classic. In this book Stephen Fry describes, in bench-top detail, techniques for the study of primary cell walls in higher plants. Deepening interest in the chelistry and metabolism of cell walls reflects the breadth of their significance in biology. Walls play crutial roles in morphogenesis, growth, disease-resistance, recognition, signalling, digestibility, nutrition and decay. Scientists interested in questions concerning these processes need to know about methods of cell wall analysis. This book was written with these people in mind. There are 11 chapters as follows: 1. Introduction to the growing plant cell wall 2. Radioactive labelling of cell walls 3. Wall polymers: extraction and fractionation 4. Wall polymers: chemical characterisation 5. Wall biosyntesis 6. Wall enzymes 7. Wall architecture 8. Wall turnover and sloughing 9. Biologically-active wall oligosaccharides 10. Wall tightening and loosening: growth 11. Wall degradation "Stephen Fry has written an invaluable book on the analytical techniques available in this important research field. This is an excellent book for the typical modern biologist." J. Experimental Biology "This book will be of considerable value to anyone engaged in the analysis of plant cell walls." Ann. Botany "For those who intend to do any practical cell wall analysis this book will be an invaluable laboratory manual." Plant Pathology "As a practical guide to wall analysis this book will be a most valuable tool." Times Higher Education Supplement

A Laboratory Guide for Cellular and Molecular Plant Biology (Biomethods)

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A Laboratory Guide for Cellular and Molecular Plant Biology (Biomethods)
I. Negrutiu , Gharti , and Chhetri
Manufacturer: Birkhauser
ProductGroup: Book
Binding: Paperback

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Book Description

This book is about spaceborne missions and instruments. In addition, surveys of airborne missions and of campaigns can be found on the accompanying CD-ROM in pdf-format. Compared with the 3rd edition the spaceborne part grew from about 300 to 1000 pages. The complete text - including the electronic-only chapters - contains more than 1900 pages. New chapters treat the history of Earth observation and university missions. The number of commercial Earth imaging missions has grown significantly. A chapter contains reference data and definitions. Extensive appendices provide a comprehensive glossary, acronyms and abbreviations and an index of sensors. An effort has been made to present the information in context, to point out relationships and interconnections. The book may serve as a reference and guide to all involved in the various national and international space programs: researchers and managers, service providers and data users, teachers and students.

Book Description

Evanescent waves play a growing role in many different areas such as guided optics, optical-fiber couplers, integrated optical elements, internal reflection spectroscopy, atom optics, dark-field microscopy, scanning tunneling optical microscopy, microaperture microscopy, and apertureless microscopies. This book describes the near field of an object through the role of the evanescent field in these areas of research. It is intended as a reference for scientists and as an introduction at the graduate level.

Nongenomic Regulation of Protein Kinase C Isoforms by Vitamin D metabolites in Chondrocyte Matrix Vesicles and Plasma Membranes

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Nongenomic Regulation of Protein Kinase C Isoforms by Vitamin D metabolites in Chondrocyte Matrix Vesicles and Plasma Membranes

Manufacturer: Storming Media
ProductGroup: Book
Binding: Spiral-bound
ASIN: 1423573196

Product Description

This is a AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH report procured by the Pentagon and made available for public release. It has been reproduced in the best form available to the Pentagon. It is not spiral-bound, but rather assembled with Velobinding in a soft, white linen cover. The Storming Media report number is A675023. The abstract provided by the Pentagon follows: Normal biologic function of an organism requires communication and regulation at several levels. Perhaps the most fundamental communication is that which occurs between the extracellular matrix and the intracellular environment. Elucidation of the signal transduction pathways in the areas of chondrocyte, osteoblast and inflammatory regulation would have significant implications in wound healing, regenerative procedures, implants and treatment of metabolic diseases of bone and cartilage. The rat costochondral chondrocyte model has been used to demonstrate that vitamin D3 metabolites can directly regulate many cellular and extracellular matrix functions and that this regulation is dependent both on the type of vitamin D metabolite, and on the maturation level of the chondrocytes. Matrix vesicles, distinct extracellular organelles critically involved in matrix mineralization, are also differentially regulated by vitamin D metabolites. Recently, it was shown that vitamin D metabolites can regulate protein kinase C activity in chondrocyte culture lysates and that these effects are also metabolite- and maturation- specific. Protein kinase C (PKC) is a family of isozymes that are known to be important in cell signal transduction. The aim of this study was to examine whether PKC mediates the nongenomic effect of vitamin D on chondrocyte regulation. The present study investigated whether PKC activity was present in matrix vesicles (MV) and plasma membranes (PM) and whether this activity can be directly regulated by vitamin D metabolites.

The Toxicology of Monoamine-Active Drugs

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The Toxicology of Monoamine-Active Drugs

Manufacturer: Storming Media
ProductGroup: Book
Binding: Spiral-bound
ASIN: 1423508009

Product Description

This is a AIR FORCE INST OF TECH WRIGHT-PATTERSONAFB OH report procured by the Pentagon and made available for public release. It has been reproduced in the best form available to the Pentagon. It is not spiral-bound, but rather assembled with Velobinding in a soft, white linen cover. The Storming Media report number is A076604. The abstract provided by the Pentagon follows: This dissertation describes a series of toxicological studies carried out in order to better understand the role of monoamine-active drugs in drug- related deaths. A simple, robust method for determining psychiatric drugs and metabolites was devised using positive mode APESI LC-MS at basic pH. This method was modified to enable determination of multiple commonly abused drugs. It was discovered that both psychiatric drugs and drugs of abuse could be detected using similar chromatographic parameters. Target drug and metabolite concentrations were determined in postmortem tissue specimens. Drug concentrations were highest in bile and liver and lowest in vitreous humour. Four patterns of distribution were observed, with extent of biliary excretion corresponding to molecular weights. Concentrations in other tissues were compared to those in blood. Several significant correlations were observed, suggesting target drug concentrations in blood could be predicted using these tissues, or could be analysed in lieu of blood in cases of extensive exsanguination or putrefaction. Antipsychotic concentrations were determined in various brain regions from schizophrenics to determine whether such drugs partition preferentially into particular regions. A further aim was to investigate whether trends exist in how they distribute in brain as a drug class. It was found that if regional drug concentrations were normalised for those in cerebellum, they distributed into three distinct patterns, corresponding to structural features of different phenothiazines.

Characterization of cyclodextrin modified infrared chemical sensors. Part II. Selective and quantitative determination of aromatic acids [An article from: Analytica Chimica Acta]

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Characterization of cyclodextrin modified infrared chemical sensors. Part II. Selective and quantitative determination of aromatic acids [An article from: Analytica Chimica Acta]
J. Yang , H.J. Lin , and H.Y. Huang
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

Elsevier | By Publisher | e-Docs | Formats | Books
ASIN: B000RR3GKM

Book Description

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
In this paper, the selectivity and sensitivity of cyclodextrin (CD) modified infrared (IR) chemical sensor in detection of aromatic acids in aqueous solutions were reported. To eliminate the interference from water, the technique of attenuated total reflection was employed. By surface treated with CD molecules on the internal reflection elements, the sensors were selective in sensing of aromatic acids compared to aromatic compounds with other functional groups. To facilitate the use of this method for the quantitative analyses of aromatic acids in aqueous solutions, analytical functions were also developed in this work and a linear relationship between analytical responses and concentrations of analytes can be obtained. To optimize the analytical conditions, the factors that influence the IR spectroscopic signals were examined. These factors included response time, CD loadings of the sensors, pH effect on response, regeneration efficiency and stability of sensors. Under the optimal conditions, the detection limits for aromatic acids at a detection time of 2min can be <100@mg/L. Meanwhile, the dynamic linear range for detection was only ca. two orders of magnitude if direct IR signals were used. Using the analytical function developed in this work, the linearity can be extended up to a concentration of 100mg/L.

Characterization of cyclodextrin-modified infrared chemical sensors [An article from: Analytica Chimica Acta]

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Characterization of cyclodextrin-modified infrared chemical sensors [An article from: Analytica Chimica Acta]
J. Yang , H.J. Lin , and H.Y. Huang
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

Elsevier | By Publisher | e-Docs | Formats | Books
ASIN: B000RR3JXG

Book Description

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
To explore the properties of cyclodextrins (CDs) as an optical sensing phase, the behavior of immobilized CD in interaction with analytes was studied in this work. CDs having different cavity sizes were immobilized onto the surface of infrared (IR) internal reflection-sensing element (IRE) to kinetically monitor the behavior of CD in interaction with analytes. Several aromatic compounds having various molecular sizes and functional groups were used to characterize the interaction mechanism. A two-layer modification method was proposed in this work, which utilized a thin hydrophobic film (polyvinyl benzyl chloride) to stick on the IRE and to covalently bond to the CDs through an ethylene diamine linker. The synthesized CD phases exhibited high stability in aqueous solution. To analyze the behavior during the formation of complexes between the guest molecules and the CD phases, we modeled the interaction behavior and treated the kinetic data with the theoretical equations developed in this work. The results indicate that the behavior of the interaction between guest molecules and CDs was explained by considering the formation of two types of complexes: adsorbed complexes and inclusion complexes. The formation of the inclusion complexes was relatively fast, the time required to reach equilibrium could be shorter than a few minutes. The adsorbed complexes were also observed, but their rate of formation was relatively slow; equilibrium could be reached at times greater than 60min. Based on the signals observed under equilibrium conditions, the concentration of inclusion complexes was approximately three times than that of the adsorbed complexes.

An evanescent wave fluorescence fiber-optic flow sensor for resin transfer molding

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An evanescent wave fluorescence fiber-optic flow sensor for resin transfer molding
Bruce K Fink
Manufacturer: Army Research Laboratory
ProductGroup: Book
Binding: Unknown Binding
ASIN: B0006RXS6K

In-Situ Kinetics of the Epoxy-Amine Interphase Curing Reaction as Revealed by FT-IR Evanescent Wave Spectroscopy

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In-Situ Kinetics of the Epoxy-Amine Interphase Curing Reaction as Revealed by FT-IR Evanescent Wave Spectroscopy
Haining Liu
Manufacturer: distributed by American Institute of Chemical Engineers]
ProductGroup: Book
Binding: Unknown Binding

Quantitative measurement of interactions between a colloidal particle and a surface using three-dimensional optical trapping and evanescent wave light scattering

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Quantitative measurement of interactions between a colloidal particle and a surface using three-dimensional optical trapping and evanescent wave light scattering
Aaron R Clapp
Manufacturer: distributed by American Institute of Chemical Engineers]
ProductGroup: Book
Binding: Unknown Binding

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Embarrassing...

Elliot's Banana

Three stories that came together to make one great novel

Not deep at all

How about alittle hot fudge with this banana?

Eliot's Banana
Heather Swain
Manufacturer: Pocket
ProductGroup: Book
Binding: Paperback

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Luscious Lemon

Cold Feet

White Bikini Panties

My Favorite Mistake

ASIN: 0743464877

Book Description

Things should be peachy. Junie isn't entirely sure what her problem is. She's just moved into a Brooklyn apartment with her cool longtime boyfriend Leon, a drummer who adores her. She flits through a string of temp jobs in funky thrift store clothes. But beneath her veneer of quirky humor there's a nagging feeling of dissatisfaction about her life. She's about to go bananas. When Junie meets Eliot, who is twice her age, and his cat, Alfie, at the vet's office, she's convinced she's found the zest missing in her life. A burnt-out sci-fi writer in search of a muse, Eliot is apples to Leon's oranges. It's not long before Junie's standing in his kitchen being offered a banana...and then some. Losing herself in the mayhem of a fling, Junie slowly realizes that kinky diversions are a poor distraction from what's really eating her. Only when she stops obsessing about Eliot and starts peeling away the layers of her family's past will she see that what she really wants has been waiting for her all along...and that her future's ripe with possibilities.

Download Description

"Things should be peachy. Junie isn't entirely sure what her problem is. She's just moved into a Brooklyn apartment with her cool longtime boyfriend Leon, a drummer who adores her. She flits through a string of temp jobs in funky thrift store clothes. But beneath her veneer of quirky humor there's a nagging feeling of dissatisfaction about her life. She's about to go bananas. When Junie meets Eliot, who is twice her age, and his cat, Alfie, at the vet's office, she's convinced she's found the zest missing in her life. A burnt-out sci-fi writer in search of a muse, Eliot is apples to Leon's oranges. It's not long before Junie's standing in his kitchen being offered a banana...and then some. Losing herself in the mayhem of a fling, Junie slowly realizes that kinky diversions are a poor distraction from what's really eating her. Only when she stops obsessing about Eliot and starts peeling away the layers of her family's past will she see that what she really wants has been waiting for her all along...and that her future's ripe with possibilities. "

Customer Reviews:

Embarrassing..........2006-04-17

Paper characters (including an unlikeable heroine), stock story, with bits of aburdities thrown in to compensate for poor writing and a plot with no momentum. In describing this book to a friend I said, "Well, it's about a girl who has an affair with a much older man, and there's a sex scene involving a banana, and a talking cat who believes that he and the main character were lovers in their prior lives as fishmongers. Or something like that." Couldn't bear to finish, but my fellow bookclubbers tell me the ending was a terrible disappointment.

Elliot's Banana.......2005-12-30

The cat was totally wierd! It was okay for a quick read but if you are looking for something deep this isn't it. I think that the author tried her best to make it something it wasn't but come on we have all the read books where someone has lost a loved one and it takes them less time to get their life back in less than 200 pages.

Three stories that came together to make one great novel.......2005-09-12

According to the Q & A by the author in this book, she originally started out writing three short stories and realized there was a way to intertwine them into this moving novel. After reading this book, I'd say she did a terrific job.

Junie knows that she's still trying to figure things out. Temp jobs by day and the girlfriend of the drummer in a local band. 25 and just moved in with Leon, the drummer. All of this fun and in NYC. Though truth be told we dont find this out about her until we meet Eliot.

Eliot, the sci-fi writer who has a cat with a story all of his own. Alfie, the cat who believes he's been reincarnated. And it is because of this cat that Eliot and Junie meet. Eliot, who is twice as old as Junie, intrigues her beyond anything she's ever known before. Junie is captivated by how quickly her life changes when she meets this writer.

The story progresses and Junie's character becomes more evident. Still burdened by the loss of her brother when she was 8, Junie believes that her whole life she's trying to find out who she is and what she's supposed to do. In the matter of one weekend, her whole perspective changes. It isn't until ending both of her relationships and attemtping to rekindle one that she thought was long gone did she realize who she was and what she was going to do.

Junie may not have things together but her transformation from taking comfort in the past to confronting her present brings a great deal of insight to this novel. The title to this book is quite appropos on many levels. From the innuendo that starts the book off and it is the understanding of how to avoid a slippery peel to confront those things in your life that trouble you.

This may not be your traditional 'chick lit' fare but it certainly is worth the read. Humor and wit, love and heartache, this novel has it all.

Not deep at all.......2005-03-24

While there were some touching moments, for the most part this book was weak. I disliked how long it took the protagonist to find out "what was wrong with her". How about some introspection? It was very obvious. Also the voice of Eliot's cat throughout was highly annoying and distracting.

How about alittle hot fudge with this banana?.......2004-12-30

I found myself wondering why Junie couldnt seem to get it right through most of this book. She seems to float along aimlessly in and out of Leons life into Eliots then back to Leon. The only thing that she seems to get any pleasure from is the banana or does she?
All in all this was a very strange book.

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Eliot's Banana
Heather Swain
Manufacturer: Simon & Schuster Adult Publishing Group
ProductGroup: Book
Binding: Mass Market Paperback
ASIN: 0641637098

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