Techniques in Quantification and Localization of Gene Expression

Book Description

The molecular basis of disease is the driving force in biomedical reserach. Gene detection, quantification, and localization are required to determine disease pathogenesis. In as much as DNA is the genetic potential in a particular organism, RNA and protein expression are the functional result of our genetic program. "Techniques in Localization of Gene Expression" describes in great detail the methods used to quantify and localize gene expression. Sensitive detection of gene expression is hindered by poor recovery or preservation of target sequences. "Techniques" emphasizes optimized methods to ensure full recovery and preservaiton of targets from specimen acquisition through detection of specific targets and final analysis. Situ Analyses of cells and tissues are also discussed. One of the weaknesses in biomedical research has been the use of in vitro model systems consisting of cell lines that may or may not mimic the in vivo conditions. These model systems are necessary to ask several questions on cell s and tissues with controllled variables int he absense of strategies to ask many questions at the same time. Multiparameter analysis can cirmcumvent the necessity fo in vitro model systems by allowing many quesitons to be answered on a cell or tissue at a single point in time. Using fluorescent dyes, on can answer many questions about a particular cell or tissue. One color, one question is an overriding theme of this book. The limitations of this approach are the number of colors in the spectrum and our ability to resolve them. Readers will find working protocols that have been developed and used routinely in the author's laboratory. Representative applications will be described using the various gene quantification and localization protocols to illustrate the utility of the techniques.

Book Description

Understanding urban air pollution is a prerequisite to finding effective solutions to air quality problems and for a sustainable development in the urban environment. In this book the current state of the art in urban air pollution research is presented. A major focus is on suitable air pollution modelling concepts, covering also street canyon geometries. Such models may be applied to establish source-receptor relationships in support of urban air quality management. Procedures for evaluating the performance of air pollution models are proposed, and results from field experiments and laboratory studies are shown to provide better insight into the characteristics of air pollution at the urban and local scales. The contents of this book are of a high policy relevance, given their direct connection to the formulation of improved tools for urban air quality assessments.

Air Quality in New York City After the September 11, 2001 Attacks: Field Hearing Before the Committee on the Environment, U.s. Senate

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Air Quality in New York City After the September 11, 2001 Attacks: Field Hearing Before the Committee on the Environment, U.s. Senate

Manufacturer: Diane Pub Co
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Air Quality in the Mexico Megacity:: An Integrated Assessment (Alliance for Global Sustainability Bookseries)

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Air Quality in the Mexico Megacity:: An Integrated Assessment (Alliance for Global Sustainability Bookseries)

Manufacturer: Springer
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Binding: Hardcover

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ASIN: 1402004524

Book Description

The quality of the air we breathe is fundamental to the quality of life for the growing millions of people living in the world's burgeoning megacities. In this book, experts in atmospheric sciences, human health, economics, social and political sciences contribute to an integrated assessment of the complex elements needed to structure air quality policy in the 21st century. The analysis is developed through a case study of the Mexico City Metropolitan Area - one of the world's largest megacities in which air pollution grew unchecked for decades. The international research team is led by Luisa T. and Mario J. Molina, Nobel Laureate in Chemistry. Improvements in Mexico City's air quality in the last decade testifies to the power of determined and enlightened policy making, and throws into relief the tough problems that remain to be solved.The volume's first six chapters, including the contributions of over 50 distinguished scholars from Mexico and the US, outline the fundamental areas of knowledge policy makers must accommodate. The message is that only good science and well-chosen technologies can direct the way to corrective regulatory measures; but without strong commitment from government, no amount of science or technology can help.In presenting what is known about the causes and consequences of air pollution in this megacity, the authors highlight what needs to be done. Many key areas of measurement and methodology for consistently and accurately assessing air quality and its effects still require refinement.The volume concludes with an extensive list of policy recommendations, emphasizing the value of integrated assessment and a long-term perspective. Intended for the guidance of policy makers in the Mexico Megacity, these recommendations form a provocative challenge to the leadership of other megacities with looming air pollution issues.While each city - its problems, resources, and perspectives - is unique, the need for an integrated assessment of complex environmental problems is the same. The case study presented in this book demonstrates ways to work toward the comprehensive knowledge needed to build robust policy.

Air Quality Management and Assessment Capabilities in 20 Major Cities (Environment Assessment Report)

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Air Quality Management and Assessment Capabilities in 20 Major Cities (Environment Assessment Report)

Manufacturer: United Nations
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Aire dividido: Critica a la politica del aire en el valle de Mexico, 1979-1996

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Aire dividido: Critica a la politica del aire en el valle de Mexico, 1979-1996
Jose Luis Lezama
Manufacturer: Colegio de Mexico, Centro de Estudios Demograficos y de Desarrollo Urbano
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Binding: Unknown Binding

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Managing Air Quality and Scenic Resources at National Parks and Wilderness Areas (A Wv Replica Edition)

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Managing Air Quality and Scenic Resources at National Parks and Wilderness Areas (A Wv Replica Edition)
R. Rowe
Manufacturer: Westview Press Inc
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Transportation, Land Use, and Air Quality: Making the Connection : Conference Proceedings May 17-20, 1998 the Benson Portland, Oregon

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Transportation, Land Use, and Air Quality: Making the Connection : Conference Proceedings May 17-20, 1998 the Benson Portland, Oregon

Manufacturer: American Society of Civil Engineers
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Urban Environmental Governance: Comparing Air Quality Management in London and Mexico City (The Political Economy of Latin America Series)

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Urban Environmental Governance: Comparing Air Quality Management in London and Mexico City (The Political Economy of Latin America Series)
Cesar Nava Escudero , and Cesar Nava Escudero
Manufacturer: Ashgate Publishing
ProductGroup: Book
Binding: Hardcover

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Weather Almanac: A Reference Guide to Weather, Climate, and Air Quality in the United States and Its Key Cities (7th ed. Issn 0731-5627)

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Weather Almanac: A Reference Guide to Weather, Climate, and Air Quality in the United States and Its Key Cities (7th ed. Issn 0731-5627)

Manufacturer: Thomson Gale
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The weather almanac;: A reference guide to weather and climate of the United States and its key cities

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The weather almanac;: A reference guide to weather and climate of the United States and its key cities
James A Ruffner
Manufacturer: Gale Research Co
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Binding: Paperback

Book Description

This volume is a translation and revision of the Original Russian version by Baryahktar. It covers all of the main fields involved in Condensed Matter Physics, such as crystallography, electrical properties, fluids, magnetism, material properties, optics, radiation, semiconductors, and superconductivity, as well as highlights of important related subjects such as quantum mechanics, spectroscopy, and statistical mechanics. Both theoretical and experimental aspects of condensed matter are covered in detail.

The entries range from very short paragraphs on topics where definitions are needed, such as Bloch's law, clathrate compound, donor, domain, Kondo lattice, mean free path, and Wigner crystal, to long discussions of more general or more comprehensive topics such as antiferromagnetism, crystal lattice dynamics, dislocations, Fermi surface, Josephson effect, luminescence, magnetic films, phase transitions and semiconductors. The main theoretical approaches to Condensed Matter Physics are explained. There are several long tables on, for example, Bravais lattices, characteristics of magnetic materials, units of physical quantities, symmetry groups. The properties of the main elements of the periodic table are given.

Numerous entries not covered by standard Solid State Physics texts
o Self-similarity
o The adiabatic approximation
o Bistability
Emphasis on materials not discussed in standard texts
o Activated carborn
o Austenite
o Bainite
o Calamitics
o Carbine
o Delat phase
o Discotics
o Gunier-Preston zones
o Heterodesmic structures
o Heusler Alloys
o Stress and strain deviators
o Vicalloy
· Each entry is fully cross-referenced to help tracking down all aspects of a topic under investigation
Highly illustrated to clarify many concepts

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Trapped

Catcher in the Rye on Crack

I don't get it

unfunny, annoyingly written

Vernon Genius Little

Vernon God Little
DBC Pierre
Manufacturer: Harvest Books
ProductGroup: Book
Binding: Paperback

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ASIN: 0156029987

Amazon.com

The surprise winner of the 2003 Man Booker Prize, DBC Pierre's debut novel, Vernon God Little, makes few apologies in its darkly comedic portrait of Martirio, Texas, a town reeling in the aftermath of a horrific school shooting. Fifteen-year-old Vernon Little narrates the first-person story with a cynical twang and a four-letter barb for each of his diet-obsessed townsfolk. His mother, endlessly awaiting the delivery of a new refrigerator, seems to exist only to twist an emotional knife in his back; her friend, Palmyra, structures her life around the next meal at the Bar-B-Chew Barn; officer Vaine Gurie has Vernon convicted of the crime before she's begun the investigation; reporter Eulalio Ledesma hovers between a comforting father-figure and a sadistic Bond villain; and Jesus, his best friend in the world, is dead--a victim of the killings. As his life explodes before him, Vernon flees his home in pursuit of a tropical fantasy: a cabin on a beach in Mexico he once saw in the movie Against All Odds. But the police--and TV crews--are in hot pursuit.

Vernon God Little is a daring novel and demands a patient reader, not because it is challenging to read--Pierre's prose flows effortlessly, only occasionally slipping from the unmistakable voice of his hero--but because the book skates so precariously between the almost taboo subject of school violence and the literary gamesmanship of postmodern fiction. Yet, as the novel unfolds, Pierre's parodic version of American culture never crosses the line into caricature, even when it climaxes in a death-row reality TV show. And Vernon, whose cynicism and smart-ass "learnings" give way to a poignant curiosity about the meaning of life, becomes a fully human, profoundly sympathetic character. --Patrick O'Kelley

Book Description

When sixteen kids are shot on high school grounds, everyone looks for someone to blame. Meet Vernon Little, under arrest at the sheriff's office, a teenager wearing nothing but yesterday's underwear and his prized logo sneakers. Moments after the shooter, his best buddy, turns the gun on himself, Vernon is pinned as an accomplice. Out for revenge are the townspeople, the cable news networks, and Deputy Vaine Gurie, a woman whose zeal for the Pritikin diet is eclipsed only by her appetite for barbecued ribs from the Bar-B-Chew Barn. So Vernon does what any red-blooded American teenager would do; he takes off for Mexico.

Vernon God Little is a provocatively satirical, riotously funny look at violence, materialism, and the American media.

Customer Reviews:

Trapped.......2007-10-06

Vernon Little lives in a small town in the middle of Texas. His friend Jesus went on a rampage, shooting fellow students at his local school. Was Vernon involved in the massacre? Did he have any influence over Jesus? The locals seem unsure. Suspicions deepen and begin to surround Vernon. Can he escape?

It's difficult to imagine a funny novel coming out of a scenario so terrible as a school massacre, yet DBC Pierre manages it. The strength of the narrative is in Vernon's frustrations at the life he leads in a stifling small town whose inhabitants add to his sense of teenage alienation. Vernon is an acerbic observer of the quirks of the middle-aged inhabitants of the town as well as the horrifically boorish and intolerant children. There's a certain Kafkaesque feeling about the novel as Vernon becomes more and more trapped by events: just as the massacre is a nightmare for the whole town, Vernon lives through a personal nightmare as the "culprit" is sought.

Along with this, of course, the novel is a satire on modern life. Although it is set in America, it observations are valid for other societies: the febrile media, spreading disinformation; rapacious money-seekers seeking to gain from the tragedy and so on.

An interesting novel.

G Rodgers

Catcher in the Rye on Crack.......2007-05-29

I hate to be another reviewer comparing Vernon God Little to Catcher in the Rye, but sometimes comparisons are inevitable, especially when a book clearly takes influence from another. Obviously Catcher is the superior book, but thta would be obvious if you were comparing Catcher in the Rye to most books. Anyway, all comparisons aside...

Vernon God Little is the story of Vernon Little who lives in a heightened version of our own reality, in a small Texas town called Martirio. Like many people Vernon hates everything about his small town, including his house, his mother, and himself. Like Holden Caulfield before him, Vernon Little spends most of this narrative novel telling us his "learnings" on various topics while making a harebrained journey to escape his life. In Vernon's case, though, he's got more reason to escape: the cops are after him.

The first quarter of this book is a little tough to get through. DBC Pierre's writing style asks a lot of the reader. Readers must learn to accept an entirely new way of plot and character development, where little plot is given and a character we don't yet know starts right in on everything he thinks about the world around him. Only about a quarter of the way in do we find out that a school shooting has happened at Vernon's school, and that his best friend Jesus was responsible. But that leaves no one alive to defend Vernon's honor and now the police see him as the number one suspect.

With a sleazy TV repairman turned news reporter on his trail, his mother always waiting for his new refrigerator, and a girl from his high school out to seduce him, Vernon has to figure out an escape plan and high tail it to Mexico.

Vernon God Little is a hilarious, highly farcical romp through many taboo subjects. DBC Pierre doesn't stop to apologize as Vernon discusses his thoughts and experiences on every subject from pedophilia to murder to bowel movements. With an out of this world cast of characters and a brilliant sarcastic narrator, the reader can't go wrong.

The first 75 pages took me a few days to get into, but the last 200 I read all in one sitting. It's that good. If you get a chance to check the book out, go for it. Like I said, it's The Catcher in the Rye on crack.

I don't get it.......2007-05-28

Remember the scene in "Big," where Tom Hanks' character comes up with a brilliant idea for a new toy and his boss couldn't be prouder? And he has this annoying colleague who keeps saying, "I don't get it?"

Learning that this book has won a major literary prize and gotten showered with accolades, makes me feel like that annoying guy. It's a coming-of-age novel that has been compared to "Catcher in the Rye", as these kinds of books invariably do. Yes, if Salinger had been bedridden for years and force fed a steady diet of American TV. It's that bad.

Vernon God Little is a teenager who has gotten mixed up in a school shooting by his best friend, now dead. He lives in one of those strenuously wacky Southern towns that only occur in literature such as this and Hollywood movies. Everyone is overweight, talks in slang, has a colorful name, and is a few sandwiches short of a picnic. Ha ha. Aren't Americans trashy? This is not exactly new news, and cariactures may be amusing but don't make a lasting impression on the reader, at least not this one.

Little's clumsy attempts to clear his name all backfire and he winds up on death row. There, he suddenly gets fed a heavy dose of life lessons. Does he die? If you make it to the end, you'll find out, but by then you might be exasperated with the book.

Authors like Jodi Picoult, who recently came out with a school shooting novel, clearly have done their homework and interviewed people who were actually involved. Although their books are fiction, they are based on real events and sound authentic.

Also there were no real sympathetic characters, unless you counted the dimbulb blond girl who liked sex. The deceased school shooter sounded as if he might have been an interesting character to flesh out more, but he remained as sketchy as the rest. The genius of "Catcher," as dated as the slang is now is the lasting impact of Holden's desire for human connection. He loved his mom, as clueless as she was, whereas Vernon regards everything his poor one does as a "knife in his back." While this may be accurate, it wound up making me more sympathetic toward his mom, not him.

unfunny, annoyingly written.......2007-03-13

I read this novel as part of my (perhaps crazy) self-imposed plan to read all the novels that have won or been shortlisted for the Booker Prize. So far, I must say that VERNON GOD LITTLE is my least favorite. The characters are cartoonish and unreal, but that's okay--many likeable novels have been told that way. The plot is contrived and unbelieveable, culminating with an absolutely preposterous, deux-a-machina ending, unredeemed, in my view, by any real charm or humor. But I suppose the facet of the book I found the most annoying was the text itself, clunky, awkward prose trying too hard to be cute. The narrator expresses himself in a way no other human ever has, and certainly no fifteen year-old American, from Texas or anywhere else. Except maybe Pluto. I think it's unwise for a writer to attempt to characterize the inhabitants of a culture foreign to his own in such a way. A brave artisic attempt? Possibly, but a failure, to me, at least. I can only assume the Booker Prize was awarded to this book more for political reasons than artistic ones--the other nominees I've read have not let me down in this way. And yes, I understand that VERNON GOD LITTLE is meant to be a satire of America's media-driven shallow consumeristic ways, but it doesn't take much talent to hit an easy target. VERNON GOD LITTLE is about as subtle as a train wreck, and every bit as funny.

Vernon Genius Little.......2007-02-18

By the time you've watched TV people be devestated because a prince decided they're not chosen to fly to Europe with him for a date, or watched them be devestated because they'll be thrown out of the Big Brother house, it's hard to feel something other than tired when the newsbreak shows people reacting to something legitimately horrible. When you watch TV humans appear to rehearse all their lives for something really bad to happen to them. Understandable, then, is Vernon's supposedly incriminating "impassiveness" after he's witnessed his best friend do a school shooting then kill himself, leaving Vernon as the "skate goat". Unlike some reviewers, I found Vernon's mix of wise and puerile and ignorant believable for a guy his age who implies a couple of times that he's "a quiet one, a wordsmith", but towards the end I felt like if he died he should've willed his memoir to his mum so she could get it published and live off the winnings from some sort of literary prize. Vernon may sound his age, but he's not an average fifteen-year-old. There's some really wonderful wordplay in this book, and only occasionally despite Vernon's poetic Vern-acular did I find myself rereading something to figure out what the hell he was on about. One star fell from my rating because on some of the rereads I found out there was no denying that I'd tripped over a strained metaphor. That, and towards the end I felt like the message was that to survive in this world you have to be a con man--"you're supposed to be a psycho". Given Pierre's background this felt a little propaganda-ish.

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Jesus Von Texas

Manufacturer: Aufbau Taschenbuch
ProductGroup: Book
Binding: Paperback

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ASIN: B000IG8G8Y

Product Description

Vernon Little sitzt im stadtischen Gefangnis von Martirio, der >> Barbecuesaucen-Hauptstadt von Texas < <. Er hat ein ernsthaftes Problem: Sein Kumpel Jesus hat soeben 16 Klassenkameraden ins Jenseits befordet und sich anschlieBend selbst erschossen. Auf Vernon konzentrieren sich nun die gesamten Rachegeluste der Stadt und die Sensationsgier der Medien.

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Vernon God Little *SIGNED 1st UK F/F*
D. B. C. PIERRE
Manufacturer: London: Faber & Faber
ProductGroup: Book
Binding: Hardcover
ASIN: B000WCVHLC

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Vernon God Little
DBC Pierre
Manufacturer: Harvest Books
ProductGroup: Book
Binding: Paperback
ASIN: B000OJKJ5I

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VERNON GOD LITTLE (CD)
Pierre & Faber & Faber
Manufacturer: Penguin Audiobooks
ProductGroup: Book
Binding: Paperback
ASIN: B000OJI6DK

Vernon God Little - A 21st Century Comedy In The Presence Of Death

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Vernon God Little - A 21st Century Comedy In The Presence Of Death
Dbc Pierre
Manufacturer: Faber And Faber
ProductGroup: Book
Binding: Paperback
ASIN: B000UKQDQA

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