Fish (Practical Approach Series)

Book Description

This convenient laboratory manual covers the theory and application of all the commonly used FISH procedures for both the research and clinical service laboratory. Readers are provided with the theoretical principles which underlie each procedure, and are then guided through the protocols in a systematic, easy to follow manner. Because FISH procedures are particularly sensitive to background and subtle variations in sample quality, the trouble-shooting sections are invaluable to the beginner interested in developing FISH capabilities in their laboratory. Areas covered include probe production and labelling; FISH gene mapping; murine, fiber, and RNA FISH; CGH and microchips; 3D FISH; SKY and M-FISH; FISH and microarrays; and FISH in clinical cytogenetics. The information is easy to follow and includes a large number of useful reference web sites in addition to a comprehensive and up to date bibliography providing background to all the advanced molecular cytogenetics techniques in current use. This book will be of particular value to scientists, technologists, and students in the areas of molecular pathology, cancer biology, genomics, haematology, oncology, clinical cytogenetics, molecular diagnostics and genetics.

Average customer rating:

Excellent book

Practical in Situ Hybridization
Trude Schwarchzacher , and Pat Heslop-Harrison
Manufacturer: BIOS Scientific Publishers
ProductGroup: Book
Binding: Paperback

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

Biotechnology | Special Topics | Medicine | Subjects | Books

ASIN: 0387915966

Book Description

In situ hybridization is a powerful technique used to characterize and locate nucleic acid sequences within the cell. These methods of molecular cytogenetics have become increasingly important over the last 10 years, and have had an important impact on many different areas of the life sciences.

Practical in situ Hybridization is aimed at researchers who wish to use efficient and reliable protocols in their work and those who need to check the validity and interpretation of published data. This book presents a reliable, tried-and-tested approach to the methodologies in current use in many laboratories. The book has a user-friendly and open format for easy access to information, comprehensive coverage of many in situ hybridization techniques, full color sections illustrating results clearly, and an extensive guides to troubleshooting

The book is an invaluable guide for all researchers who need to understand the principles and practice of in situ hybridization.

Customer Reviews:

Excellent book.......2001-01-04

A real treat for Insitu field. Describes the procedure from step one to the end. Good for both beginers and experienced workers. Only complete book available in the field.

PCR 3: PCR In Situ Hybridization: A Practical Approach

Average customer rating:

PCR 3: PCR In Situ Hybridization: A Practical Approach
C.S. Herrington
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

Genetics | Evolution | Science | Subjects | Books

General | Science | Subjects | Books

Natural History | Nature & Ecology | Science | Subjects | Books

Genetics | Basic Science | Medicine | Subjects | Books

Book Description

PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.

In Situ Hybridization: A Practical Approach

Average customer rating:

Exellent and newest protocol

In Situ Hybridization: A Practical Approach
D.G. Wilkinson
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

Genetics | Evolution | Science | Subjects | Books

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Natural History | Nature & Ecology | Science | Subjects | Books

ASIN: 0199636591

Book Description

In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

Customer Reviews:

Exellent and newest protocol.......2000-10-11

This book gives newest and exellent protocol. Contents are not so big but enough detail to follow experiments.

Molecular Imaging in Neuroscience: A Practical Approach (Practical Approach Series)

Average customer rating:

Molecular Imaging in Neuroscience: A Practical Approach (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

General | Science | Subjects | Books

Book Description

This book reflects the recent dramatic advances in the use of modern imaging techniques to visualize and quantify the various components of cell structure and cell functional activity in the nervous system. Readers will find detailed protocols for imaging neurotransmitter and receptor mRNAs
using radioactive and non-radioactive in situ hybridization technology. Methods for visualizing transmitter and drug receptors using quantitative autoradiography, sequence-specific antibodies, and immunocytochemistry are also clearly described. Plus, the book covers the imaging of biological
activity in living cells, including methods of monitoring intracellular pH and calcium mobilization using selective ion-sensitive dyes in conjunction with flow cytometry and video microscopy. This is an essential guide for researchers wanting to exploit these powerful new techniques for their
studies in neuropharmacology, neuroanatomy, neurogenetics, neuropathology, and pharmaceutical research. receptors, and receptor function in the nervous system. They represent the state of the art for studying brain function and dysfunction in the laboratory. It will be of major interest to
neuroscientists and pharmacologists.

Tissue In Situ Hybridization: Methods in Animal Development (An Embo Practical Course)

Average customer rating:

Tissue In Situ Hybridization: Methods in Animal Development (An Embo Practical Course)
Trevor Jowett
Manufacturer: Wiley
ProductGroup: Book
Binding: Paperback

Experiments & Projects | Experiments, Instruments & Measurement | Science | Subjects | Books

General | Science | Subjects | Books

Natural History | Nature & Ecology | Science | Subjects | Books

Biotechnology | Special Topics | Medicine | Subjects | Books

General | Research | Medicine | Subjects | Books

Research | Medical | Professional & Technical | Subjects | Books

All Titles | Qualifying Textbooks - Fall 2007 | Stores | Books
ASIN: 0471164038

Book Description

TISSUE IN SITU HYBRIDIZATION

Methods in Animal Development

Trevor Jowett

The European Molecular Biology Organization (EMBO) course on tissue in situ hybridization in animal developmental biology has served as an important and highly respected forum for the latest advances in the methodology of this valuable research tool. Developed from EMBO course materials, Tissue

In Situ Hybridization provides scientists and researchers worldwide with detailed coverage of new approaches, techniques, and protocols, along with up-to-date information on more established procedures.

Focusing particularly on the two-color in situ hybridization method to whole-mount embryos and tissue sections, this practical resource also compares different methods of producing differentially colored signals, and includes the results of protocol experiments with fluorescent and other alternative in situ hybridization techniques. Special features include:

Photographic examples—including color plates—to complement the text
Clear explanations of the principles underlying different methods
Detailed discussion and comparison of the different methods
Valuable troubleshooting advice and practical guidance
Comprehensive index, allowing quick and easy access to specific topics

Compiled by a leading expert in the field, Tissue In Situ Hybridization is an indispensable asset to professionals and researchers working in the areas of developmental, cell, and molecular biology.

Average customer rating:
In Situ Hybridization: A Practical Approach
D. G. (EDT) Wilkinson
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback
ASIN: B000OKYZTI

Book Description

KEY BENEFIT: Now in its third edition, this book teaches physical concepts using computer simulations. The text incorporates object-oriented programming techniques and encourages readers to develop good programming habits in the context of doing physics. Designed for readers at all levels , An Introduction to Computer Simulation Methods uses Java, currently the most popular programming language. Introduction, Tools for Doing Simulations, Simulating Particle Motion, Oscillatory Systems, Few-Body Problems: The Motion of the Planets, The Chaotic Motion of Dynamical Systems, Random Processes, The Dynamics of Many Particle Systems, Normal Modes and Waves, Electrodynamics, Numerical and Monte Carlo Methods, Percolation, Fractals and Kinetic Growth Models, Complex Systems, Monte Carlo Simulations of Thermal Systems, Quantum Systems, Visualization and Rigid Body Dynamics, Seeing in Special and General Relativity, Epilogue: The Unity of Physics For all readers interested in developing programming habits in the context of doing physics.

Customer Reviews:

Pretty good introductory book.......2005-10-19

Good introductory book on Computational Physics.
Projects proposed cover different difficulties so it can be used for college students at all levels.Concepts are well explainded. Introduces areas including linear and nonlinear systems, normal modes and waves, and electrodynamics. This second edition offers expanded material on chaos, complexity, and quantum mechanics, programs with more use of graphics, and appendices on Fortran and C.

very good introductory computational physics book.......2005-04-28

This was my first comp. physics book and it was easy and
very instructive. I can assure your money is well spent.
If you find this book too easy, then you should move on to
Thijssen's Computational Physics which is intended for the
`graduate' level readers.
Only 4 stars (not 5) because I don't think that the codes
provided in the book are that useful. Everyone has different
coding style. What really matters is the physical concepts and
not the numerical receipes.

Makes Physics a pleasure.......2004-04-19

Excellent, outstanding book for clarity, elegance, writing style and level of detail. This book won't leave you alone on the intricacies of marrying physics and computer programming and will make your programming activity more intereesting if you are more of a programmer and you physical studies more rewarding and less
"pen-and-paperlike" if you are an aspiring physicist. In fact I highly recommend this book to anyone that has an interest in how nature works and in playing with computers. The extreme clarity of this book makes it accessible to junior colege students and even to advanced, motivated high school students or for self study. I would have preferred to see the example code in the book
in C or better C++ but using BASIC has the advantage of making the book more esy to read and is just sligtly annoying for the
C++ or Java programmer. ( Are there any other kind of real programmers? ;) Just make sure you master your language enough to make calculations, use functions, and are able to produce at least some basic graphics (using C++ and openGL or DirectX would rock). Judge by yourself, but I would buy without a thought a book whose first chapter is on the "Coffe Cooling Problem".

An Introduction to Computer Simulation Methods Applications to Physical Systems: Part II (Introduction to Computer Simulation)

Average customer rating:

An Introduction to Computer Simulation Methods Applications to Physical Systems: Part II (Introduction to Computer Simulation)
Harvey Gould , and Jan Tobochnik
Manufacturer: Addison-Wesley
ProductGroup: Book
Binding: Paperback

General | Computers & Internet | Subjects | Books

An Introduction to Computer Simulation Methods : Applications to Physical Systems Patr 2.

Average customer rating:

An Introduction to Computer Simulation Methods : Applications to Physical Systems Patr 2.
Harvey and Tobochnik , Jan Gould
Manufacturer: Addison Wesley
ProductGroup: Book
Binding: Paperback
ASIN: B000H4FEFU

Average customer rating:

An Introduction to Computer Simulation Methods: Applications to Physical System
Harvey, Tobochnik, Jan Gould
Manufacturer: Addison Wesley
ProductGroup: Book
Binding: Paperback
ASIN: B000OP1KLE

Book Description

Seneca is a figure of first importance in both Roman politics and literature: a leading adviser to Nero who attempted to restrain the emperor's megalomania; a prolific moral philosopher; and the author of verse tragedies that strongly influenced Shakespeare and other Renaissance dramatists. Here is the first of a new two-volume edition of Seneca's tragedies, with a fully annotated translation facing the Latin text.

Seneca's plays depict intense passions and interactions in an appropriately strong rhetoric. Their perspective is much bleaker than that of his prose writings. In this new translation John Fitch conveys the force of Seneca's dramatic language and the lyric quality of his choral odes.

Books:

Books Index

Books Home

Recommended Books