Culture of Epithelial Cells

Book Description

"...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years."
—Pharmaceutical Research

This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.

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Wonderful Compendium.......2002-11-26

Epithelial Cell Culture (Handbooks in Practical Animal Cell Biology)

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Epithelial Cell Culture (Handbooks in Practical Animal Cell Biology)

Manufacturer: Cambridge University Press
ProductGroup: Book
Binding: Paperback

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ASIN: 052155991X

Book Description

Epithelial Cell Culture contains chapters by experts on epithelial cells derived from the airway, intestine, pancreas, kidney and bladder, genital ducts, mammary glands, skin glands and appendages, and keratinocytes.

Epithelial Cell Culture: A Practical Approach

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Epithelial Cell Culture: A Practical Approach

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Paperback

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ASIN: 0199635722

Book Description

Cell culture has progressed to the point where cells can be grown into tissue-like structures in vitro, but these tissues often bear little resemblance to the same tissue in a living organism. This book describes methods of culturing epithelial cells so as to mimic accurately their behaviour
in vivo, and the resulting model systems can be used in a wide range of research areas: pharmaceutics, pharmacology, toxicology, and cell biology. Written by an international panel of researchers, the book details the methods used to reconstruct epithelia in vitro, the procedures used to
characterise these model systems, and the research applications for which these cultures are becoming so important.

Cell Culture Models of Biological Barriers: In vitro Test Systems for Drug Absorption and Delivery

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Cell Culture Models of Biological Barriers: In vitro Test Systems for Drug Absorption and Delivery

Manufacturer: CRC
ProductGroup: Book
Binding: Hardcover

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ASIN: 0415277248

Book Description

Over the past ten years several sophisticated in vitro test systems based on epithelial cell cultures have been introduced in the field of drug delivery. These models have been found to be very useful in characterizing the permeability of drugs across epithelial tissues, and in studying formulations or carrier systems for improved drug delivery and enhanced absorption. Compared to in vivo trials on animals or humans, cell culture models are faster, more convenient and cost effective, ethically advantageous and, most importantly, they can be more easily standardized and validated. This book provides a practical approach to contemporary cell culture-based in vitro techniques for drug transport studies at biological absorption barriers. It is an invaluable source of information for students attending graduate courses on this subject and pharmaceutical scientists working in industry or in academia.

Epithelial Cell Culture Protocols (Methods in Molecular Biology)

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Epithelial Cell Culture Protocols (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896038939

Book Description

Well-versed experimenters and clinical researchers share their best methods for establishing and maintaining epithelial cell cultures, for analyzing and studying their characteristics, and for using them to set up models of critical biological systems. The emphasis is on the analysis and assessment of epithelial cells, for example by looking at apoptosis and integrins, or by measuring membrane capacitance and confluence. Also described in step-by-step detail are co-culture techniques valuable in developing models for investigating many different in vitro systems, including the blood-brain barrier, drug uptake, and the interaction of epithelial cells with bacteria. Epithelial Cell Culture Protocols offers a step-by-step guide toward a deeper understanding of cellular and molecular mechanisms, as well as a set of robust techniques for specifically evaluating the nature and behavior of epithelial cells.

Functional Epithelial Cells in Culture (UCLA Symposia on Molecular and Cellular Biology)

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Functional Epithelial Cells in Culture (UCLA Symposia on Molecular and Cellular Biology)

Manufacturer: Alan R. Liss
ProductGroup: Book
Binding: Hardcover

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Methods for serum-free culture of epithelial and fibroblastic cells (Cell culture methods for molecular and cell biology)

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Methods for serum-free culture of epithelial and fibroblastic cells (Cell culture methods for molecular and cell biology)

Manufacturer: A.R. Liss
ProductGroup: Book
Binding: Unknown Binding

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ASIN: 0845138022

Small Bowel Enterocyte Culture and Transplantation (Medical Intelligence Unit)

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Small Bowel Enterocyte Culture and Transplantation (Medical Intelligence Unit)
Frederick C. Campbell
Manufacturer: R. G. Landes
ProductGroup: Book
Binding: Hardcover

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Tissue Culture of Epithelial Cells

Manufacturer: Springer
ProductGroup: Book
Binding: Hardcover

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Drugs impair keratinocyte DNA repair in culture.(Clinical Rounds) : An article from: Skin & Allergy News

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Drugs impair keratinocyte DNA repair in culture.(Clinical Rounds) : An article from: Skin & Allergy News
Patrice Wendling
Manufacturer: Thomson Gale
ProductGroup: Book
Binding: Digital
ASIN: B000BNFMOA
Release Date: 2005-10-04

Book Description

This digital document is an article from Skin & Allergy News, published by Thomson Gale on July 1, 2005. The length of the article is 618 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.

Citation Details
Title: Drugs impair keratinocyte DNA repair in culture.(Clinical Rounds)
Author: Patrice Wendling
Publication: Skin & Allergy News (Magazine/Journal)
Date: July 1, 2005
Publisher: Thomson Gale
Volume: 36 Issue: 7 Page: 42(1)

Distributed by Thomson Gale

Book Description

Teaches the fundamentals of the BASIC programming language by description and example, and presents over 50 chemically oriented BASIC programs that can both teach about the language and be useful in their own right. The first part of the book introduces the reader to programming in the BASIC language. The second part of the book consists of 52 example problems, divided into 44 topics, concerning chemical problems. These problems progress in difficulty in terms of the chemical concepts, mathematical models, and programming operations involved. The reader can work the problems, then copy and run the programs, and compare the results. The given programs can be modified to suit the reader's needs, or new ones be written using the techniques presented in the text.

Introduction to computer programming for chemists

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Introduction to computer programming for chemists
Thomas L Isenhour
Manufacturer: Allyn and Bacon
ProductGroup: Book
Binding: Unknown Binding

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Introduction to computer programming for chemists: FORTRAN

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Introduction to computer programming for chemists: FORTRAN
Thomas L Isenhour
Manufacturer: Allyn & Bacon
ProductGroup: Book
Binding: Unknown Binding

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Book Description

Volume 2: Superstring Theory and Beyond, begins with an introduction to supersymmetric string theories and goes on to a broad presentation of the important advances of recent years. The book first introduces the type I, type II, and heterotic superstring theories and their interactions. It then goes on to present important recent discoveries about strongly coupled strings, beginning with a detailed treatment of D-branes and their dynamics, and covering string duality, M-theory, and black hole entropy, and discusses many classic results in conformal field theory. The final four chapters are concerned with four-dimensional string theories, and have two goals: to show how some of the simplest string models connect with previous ideas for unifying the Standard Model; and to collect many important and beautiful general results on world-sheet and spacetime symmetries.

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The definitive introduction to String Theory.......2007-02-27

I used Polchinski Vol I and II to learn String Theory for my PhD thesis. Vol I covers the basics, using the bosonic string to quantize, and Vol II starts off with the perturbative type II and heterotic strings and soon moves on to nonperturbative string theory, elegantly summarizing the dualities, D-branes (which of course Polchinski discovered), Calabi-Yau compactifications, flop transitions etc. Vol II is essentially a reference for modern string theory. The chapter on advanced CFT is wonderfuly written, and is perhaps the best summary of CFT available in any book (even tomes devoted to CFT pale in comparison). The two volumes of Polchinski are clearly a must read for anybody who wishes to understand string theory. It is an exceptionally well written text on a difficult subject.

Great book, wish there was more.......2006-04-01

This book picks up for Volume I left off. Supersymmetry is added to strings, more symmetries are presented, string theory phenomenology is described and many topics introduced in Volume I are developed in more detail. The quality is as good as it was in the previous volume and I think this book is essential reading for anyone attempting to master string theory.

The main addition in this volume is the expanded coverage of supersymmetry. In brief, the early chapter topics include the relation of worldsheet supersymmetry to spacetime supersymmetry, the various types of superstrings (I, IIA, IIB, and Heterotic) and even W-algebras. Calculations of critical dimensions, scattering amplitudes and anomaly cancellations are also done.

After incorporating supersymmetry into D-branes and T-duality (two concepts first introduced in the context of bosonic strings in Volume I), the text moves on to cover strings at strong coupling. It's a great chapter, my favorite of the book. Among the things covered are U-duality, black hole entropy, the black hole information paradox and M-theory.

After a chapter covering conformal field theory in more detail there are two chapters on compactification. One for orbifolds and one for Calabi-Yau spaces. The latter gives a nice overview of the techniques of complex geometry and algebraic topology needed to study Calabi-Yau spaces (there is also an appendix covering much of the math). Phenomenology is touched on in these, but it's in the following chapter that a more fully developed presentation of the possible string theory connection with the standard model and grand unified field theory is given. One thing I enjoyed was that in addition to string specific physics, this chapter discusses more general aspects of grand unified theories.

The final chapter contains a collection of advanced topics, with mirror symmetry being one of them.

To summarize this is a can't miss book for people seeking to understand string theory. Is anything missing? Not really, although more extensive coverage of black holes and D-branes would've been nice. It definitely left me wishing there was a Volume III on the horizon.

Fair exposition.......2001-07-22

In the second volume of the series the author generalizes the results of the first to string theories where supersymmetry is present. The mathematics introduced is non-rigorous, and the strategy is to see how much of the formalism for the bosonic case can be carried over to the case where fermions are present. The book is purely an exposition on the subject of string theory, and so no attempt is made to give the reader an in-depth explanation of the ideas and concepts in this area. This is particularly noticeable in the chapter on Calabi-Yau compactification and in the discussion on mirror symmetry in the last chapter. Here is a brief outline of the contents of the book:

- Generalize the mass-shell condition (Klein-Gordon equation in momentum space) by using the Dirac equation.

- The gamma matrices will serve as CM modes of an anticommuting world sheet field.

- The resulting world-sheet supercurrents generate the superconformal transformations of the superconformal algebra.

- Counting the number of (3/2, 0) currents classifies the different superconformal field theories.

- Standard quantization techniques for constrained systems are applied.

- Free SCFTs can be obtained with the vanishing of the central charge giving 10 as the critical dimension.

- SCFT on a circle gives two periodicity conditions for the matter fermions (Ramond and Neveu-Schwarz sectors).

- Ramond and Neveu-Schwarz algberas result. - Holomorphicity constraints give bosonization via the relation between the R sector vertex operators and bosonic winding state vertex operators.

- In 10 flat dimensions, 16 sectors result from the R and NS sectors, 6 of which are empty.

- Consistency conditions yield type IIA and IIB superstring theories.

- The vacuum amplitude for a closed superstring can be found by imposing modular invariance.

- Divergences cancell in the cylinder, Mobius strip, and Klein bottle graphs.

- Generalize preceding constructions by looking for sets of holomorphic and antiholomorphic currents whose Laurent coefficients form a closed algebra.

- Consider algebras that are different on the left- and right-moving sides of the closed string, obtaining the heterotic string.

- Setting the dimensions to be the same at each side and 32 left-moving spin-1/2 fields gives the SO(32) string.

- Split these fields into sets of 16 with independent boundary conditions to get the E8 X E8 heterotic string.

- Use supersymmetry constraints to study interactions of massless degrees of freedom.

- Tree-level interactions can be studied within low-energy supergravity; one-loop gives rise to anomalies.

- Anomalies cancell in type IIA, IIB, type I, and heterotic string theories.

- Use string perturbation theory to calculate amplitudes and interactions.

- Introduce supersymmetry in toroidally compactified string theory, to obtain D-branes which are BPS states and carry R-R charges.

- Type I, IIA, IIB string theories become states in a single theory.

- Study strongly coupled strings using D-brane states.

- The five string theories are limits of a single theory in 11-dimensional spacetime.

- Study conformal field theories as a prolegomena to analyzing string compactification.

- Study string compactification via free world-sheet conformal field theories or interacting exactly solvable conformal field theories.

- Connect the compactified string theory to the Standard Model.

- Start with orbifolds and then the more general Calabi-Yau manifolds.

- Techniques from algebraic geometry are brought in to study the properties of Calabi-Yau manifolds.

- Deduce an effective (low-energy) four-dimensional action using the topology of Calabi-Yau manifolds.

- Elaborate on the physics of four-dimensional string theory.

- Try to deal with the strong CP problem using Peccei-Quinn symmetry and the resulting axion field.

- Try to understand how gauge symmetries arise in the different string theories and how they are related to the ones in the Standard Model.

- Try to connect the different mass scales in string theory.

- Study more advanced topics in string theory, such as N = 2 superconformal algebras, type II superstrings on Calabi-Yau manifolds, string theories on the 4-dimensional Calabi-Yau manifold K3, minimal models, and mirror symmetry.

- Mirror manifolds can be constructed explicitly using Gepner models.

- Use mirror symmetry to obtain the full low energy field theory at the string tree level.

- Flop transitions can occur in string theory, giving dynamical changes in topology.

Currently one of the standards.......2001-03-14

Polchinski's book on string theory is a very well written book about the subject. Also, the problems given in the book are valuable for a further understanding. Using it together with the book by Green, Schwarz, Witten one will afterwards have indeed little problems understanding the papers on this subject. However one caveat: if one reads this book, he or she shoudl be always aware that this topic is still deeply a research subject and by no means settled like mechanics. If this is always kept in mind, then this book is of considerable help in understanding one of the current frontiers of physics.

Perfect book!.......1999-06-25

Reading this book is the easiest way to become familiar with various topics that seemed to be extremely difficult before. The reader then shouldn't have any problems with understanding current research papers.

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