Aqueous Two-Phase Systems (Methods in Enzymology)

Book Description

This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells.

Key Features
* Use of affinity partitioning for enzyme purification
* Separation and study of cells
* Isolation of plasma membranes
* Large scale biotechnical use

Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology)

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Aqueous Two-Phase Systems: Methods and Protocols (Methods in Biotechnology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 0896035417

Book Description

Rajni Hatti-Kaul and her expert coauthors combine theory, methodology, and applications in a practical collection of easily reproducible protocols for bioseparations in aqueous two-phase systems (ATPS). The protocols range from established methods to cutting-edge techniques with potential biotechnological applications. Among the methods detailed are those for ATPS preparation and characterization, for partitioning applied to soluble molecules and particulates (including whole cells, membranes, and organelles), and for the isolation and purification of proteins-including a glimpse of large-scale handling of two-phase separations. Practical and informative, with its detailed guidelines allowing researchers to adapt specific systems to their own separation needs, Aqueous Two-Phase Systems: Methods and Protocols demonstrates the scope and utility of two-phase aqueous systems in both basic and applied research.

Partition of Cell Particles and Macromolecules: Distribution and Fractionation of cells, viruses, microsomes, proteins, nucleic acids, and antigen-antibody complexes in aqueous polymer two-phase Systems

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Partition of Cell Particles and Macromolecules: Distribution and Fractionation of cells, viruses, microsomes, proteins, nucleic acids, and antigen-antibody complexes in aqueous polymer two-phase Systems
Per-Ake Albertsson
Manufacturer: John Wiley & Sons
ProductGroup: Book
Binding: Hardcover
ASIN: B000HA0BDO

Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology

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Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology
Harry Walter
Manufacturer: Academic Press
ProductGroup: Book
Binding: Hardcover

Biochemistry | Biological Sciences | Science | Subjects | Books

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Extraction of testosterone and epitestosterone in human urine using 2-propanol-salt-H"2O system [An article from: Analytica Chimica Acta]

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Extraction of testosterone and epitestosterone in human urine using 2-propanol-salt-H"2O system [An article from: Analytica Chimica Acta]
H. Liu , C. He , D. Wen , F. Liu , and K. Li
Manufacturer: Elsevier
ProductGroup: Book
Binding: Digital

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ASIN: B000RR709K

Book Description

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Based on the 2-propanol-salt-H"2O system, a new and simple pretreatment method was developed for the analysis of testosterone and epitestosterone in human urine coupled with high-performance liquid chromatography (HPLC). The influence factors on partition behaviors of the steroids were studied including the type and amount of salts, the volume of 2-propanol, the concentration of the analytes, and temperature. When K"3PO"4, K"2HPO"4, K"2CO"3 and KOH were chosen as phase separation salts, the enrichment factors were 12, 10, 12 and 13, respectively, and the extraction efficiencies for testosterone and epitestosterone were all 80-90%. In the optimal system of 2-propanol-K"3PO"4-H"2O, the recoveries of the spiked standards for the analytes were all 92-102% with relative standard deviation of 2.3-7.2%. Combined with a HPLC method, this extraction technique has been successfully applied to the analysis of testosterone and epitestosterone in human urine with the detection limits of 1ng/ml and the linear ranges of 10-500ng/ml for both compounds. Compared with conventional liquid-liquid extraction or solid-phase extraction, this novel method is much simpler and more environmentally friendly due to the direct injection of the upper phase into HPLC system, no use of harmful organic solvent, and low consumption of extractant.

Aqueous Two-Phase Systems (Methods in Enzymology)

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Aqueous Two-Phase Systems (Methods in Enzymology)
Harry Walter
Manufacturer: Academic Press
ProductGroup: Book
Binding: Paperback
ASIN: B000OH54PA

Demixing of aqueous polymer two-phase systems in low gravity (SuDoc NAS 1.15:101141)

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Demixing of aqueous polymer two-phase systems in low gravity (SuDoc NAS 1.15:101141)
NASA
Manufacturer: National Aeronautics and Space Administration
ProductGroup: Book
Binding: Unknown Binding
ASIN: B0001043PC

Enhancing the Partition Coefficients of Proteins in Aqueous Two-Phase Systems with Fusion Handles

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Enhancing the Partition Coefficients of Proteins in Aqueous Two-Phase Systems with Fusion Handles
Mark A Eiteman
Manufacturer: distributed by American Institute of Chemical Engineers]
ProductGroup: Book
Binding: Unknown Binding

Molecular Thermodynamics of Phase Equilibria for Aqueous Two-Protein Systems

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Molecular Thermodynamics of Phase Equilibria for Aqueous Two-Protein Systems
Camille O Anderson
Manufacturer: American Institute of Chemical Engineers
ProductGroup: Book
Binding: Unknown Binding

$Partition of cell particles and macromolecules;: Distribution and fractionation of cells, mitochondria, chloroplasts, viruses, proteins, nucleic acids, ... in aqueous polymer two-phase systems$
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Partition of cell particles and macromolecules;: Distribution and fractionation of cells, mitochondria, chloroplasts, viruses, proteins, nucleic acids, ... in aqueous polymer two-phase systems
Per Ake Albertsson
Manufacturer: Wiley-Interscience
ProductGroup: Book
Binding: Unknown Binding

Natural History | Nature & Ecology | Science | Subjects | Books
ASIN: B0006C2XVQ

Book Description

Lewis Carroll Epstein explains deep ideas in physics in an easy-to-understand way. Thinking Physics is a perfect beginner’s guide to an amazingly wide range of physics-related questions. The book targets topics that science teachers and students spend time wondering about, like wing lift. Epstein elucidates the familiar but misunderstood — such as how tides work — along with more obscure but fascinating phenomena like the “Bernoulli sub” and the “artificial aurora” created by hydrogen bombs. Broken into many short sections and peppered with Epstein’s own playful hand-drawn illustrations, the book does not simply give the right answer: It also goes into the answers that seem right but are wrong and shows why they are wrong — a rarity in science books. Thinking Physics is a rigorously correct, lighthearted, and cleverly designed Q and A book for physicists of all ages.

Customer Reviews:

A must for every teacher.......2007-07-16

Simple, but non trivial, conceptual problems with clear, insightful, elegant solutions without math. For everyday use to challenge students' minds.

Really makes you think........2007-01-10

Think you know physics? The simple questions posed in this book will show you if you do. It is approachable enough for a lay person to pick it up and take a crack at it. The solutions give great insight for the seasoned physicists and newbies.

Elegant, brilliant answers.......2005-09-16

Epstein presents a bunch of physics problems, and solves them all -- almost without any math -- with clear, insightful, elegant solutions. A brilliant book useful from grade 10 to grad school.

Very Useful Book.......2004-05-14

No, this isn't a perfect book. Some of Lewis' explanations are a bit vague and some are incomplete but despite these minor flaws this book does a wonderful job of explaining important concepts of physics in very simple terms.

I can't think of anyone who wouldn't benefit from reading this book. Oh yes, it's a fun read too!

teachs you how to think about physics problems.......2003-12-03

I like this book because it has interesting and motivating physics problems that can be solved without math, but with just carefull thinking on the truly physical aspects and principles. I do not mean that math should not be used in physics books, nor that all physics problems can be solved without math, but problems that do not need math are also very interesting and should be taught; this book is very good in doing that. I think this book would be very useful in high school (as complement to conventional books) because it would have great effect on kids's mental habits and rational thinking.

I am sure that if you are not a professional physicist but you like physics, like me, you will enjoy with this book. The only one thing I do not like from this book is that answers are written upside down. It deserves 5 stars.

Book Description

Nestled in the rain shadow of Vancouver Island, the Gulf Islands bask in the mildest climate in Canada, a mini-Mediterranean that fosters an abundance of plant and animal species seen nowhere else. Throw in panoramic views, inviting beaches and friendly hospitality - all just a short ferry ride from Vancouver or Victoria - and you have a hiker's paradise.

Whether your visit lasts a day or a week, Hiking the Gulf Islands is the best guide to exploring the islands on foot. It is the only comprehensive guide with up-to-date information on the recently created Gulf Islands National Park - 33 square kilometres scattered over 16 islands and more than 30 islets and reefs. Separate chapters cover each island - including the often-overlooked Texada, Lasqueti and Thetis islands plus Bowen Island at the entrance to Howe Sound - and even hiking trails in the area's marine parks.

Hiking the Gulf Islands includes:
* Maps showing trail locations.
* Distance and time of each hike.
* Accessibility and degree of difficulty of each route.
* Lists of shore and road walks.
* Paddling suggestions for kayak or canoe.
* What to bring and what to look for.

THE ENCHANTED ISLE (Originally Published in the USA as BLOW THE WIND SOUTHERLY)

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A wonderful story!

THE ENCHANTED ISLE (Originally Published in the USA as BLOW THE WIND SOUTHERLY)
D. E. Stevenson
Manufacturer: Ace Books
ProductGroup: Book
Binding: Mass Market Paperback
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ASIN: 0441205917

Customer Reviews:

A wonderful story!.......2004-03-25

Originally published in the U.S. as "Blow the Wind Southerly". This is very hard to find, but worth the search. Charlotte Fairlie was proud of her position as headmistress of Saint Elizabeth's-even if it meant a lonely life for someone so young and attractive. School intrigues and petty jealousies made it imperative that she not play favorites, and forced her to remain aloof...and alone! Then she decides to accept a student's invitation to visit her on a beautiful island off the coast of Scotland, and Charlotte finds herself falling in love with a handsome laird. But an ancient legend and a living girl become obstacles to an idyllic romance. Believable and an easy read.

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Melville's Inferno

The Enchanted Isles (Hesperus Classics)
Herman Melville
Manufacturer: Hesperus Press
ProductGroup: Book
Binding: Paperback

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ASIN: 1843910152

Book Description

One of Melville’s earliest writings, The Enchanted Isles is a hauntingly beautiful descriptive work inspired by a tour of the Galapagos Islands. Originally penned as a travelogue, Melville’s writings form a series of evocative sketches of each of the Galapagos Islands. Recounting their myths and legends, he conjures up the spirit of each in astonishing detail. Read together, they create a timeless classic, perfectly capturing the islands’ eternal charm and mystery.

Customer Reviews:

Melville's Inferno.......2003-09-27

15 years ago I read all of Melville's novels and most of his other prose, but I must have missed this one. So along comes this handsome edition of the sketches and narratives about the Encantadas (Darwin's Galapagos)- and not an early work, but written after Moby Dick - which in less than a hundred pages shows once again what an astounding writer Melville is. The four pages devoted to the island's tortoises are as good as any he wrote about whales. A sample: "That these tortoises are the victims of a penal, or malignant, or perhaps a downright diabolical enchanter, seems in nothing more likely than in that strange infatuation of hopeless toil which so often possesses them. I have known them in their journeyings ram themselves heroically against rocks, and long abide there, nudging, wriggling, wedging, in order to displace them, and so hold on their inflexible path. Their crowning curse is their drudging impulse to straightforwardness in a belittered world." Of the actual narratives, the most anthologized is the story about the Chola Widow, which is heartbreaking, but even more so when read in the context of the whole suite of pieces. If you think you won't like Melville, try this book; and if you do like Melville, don't miss it.

Enchanted isles (Treasury of literature readers ; Read text series)

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Enchanted isles (Treasury of literature readers ; Read text series)
Eleanor M Johnson
Manufacturer: C.E. Merrill Books
ProductGroup: Book
Binding: Unknown Binding

General | Short Stories | Literature & Fiction | Subjects | Books
ASIN: B0007F36R2

Lions in the New Land: The Epic Adventures of Friar Nicholas in the Enchanted Isles

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Lions in the New Land: The Epic Adventures of Friar Nicholas in the Enchanted Isles
Gunnar Thompson
Manufacturer: Misty Isles Press
ProductGroup: Book
Binding: Paperback

Contemporary | General | Literature & Fiction | Subjects | Books
ASIN: 0962199079

Book Description

In Thompson's latest book, Lions in the New Land: The Epic Adventures of Friar Nicholas in The Enchanted Isles, he shares the story of a mysterious English friar whose passage through time altered the course of world history. In the 14th century, the friar traveled from England, to Norway, Iceland, and the Northern Regions in the service of King Edward III. The friar's assignment was to survey isles of the North Atlantic in an effort to find the lost, overseas colony of King Arthur. This project, entailing discovery of the Western Hemisphere, was first proposed by England's premier scientist—Roger Bacon in 1266.

We now have evidence in the form of a map and a manuscript, the Inventio Fortunatae, confirming that English Franciscans traveled to the New World at the very dawn of the Age of Discovery. At this point in time, the "New Land" was claimed as a sovereign realm under the aegis of King Magnus Eriksson—ruler of both Norway and Sweden. It is also clear that the Friar's Map played a crucial role in early Portuguese and Spanish navigation.

The manuscript reached the hands of Portugal's Prince Henry The Navigator who was the grandson of the English regent John of Gaunt, Duke of Lancaster and son of Edward III. The Inventio Fortunatae was also known to Bristol merchants who sailed to Newfoundland in the 1480's, and it was known to Columbus who reviewed the book prior to his Arctic voyage in 1477 and his Venezuelan voyage in 1498. A map of Arctic regions attributed to the friar appeared in the works of such renowned geographers as Martin Behaim, John Ruysch, Ortelius, and Mercator.

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