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DNA and Protein Sequence Analysis: A Practical Approach
Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover
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ASIN: 0199634645 |
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Sequence data--either lists of nucleotides or of amino acids--are now easily gathered using automated equipment; the real effort is involved in interpreting the data to produce predictions of protein structure or function. With the advent of worldwide computer networks, a plethora of software
is now available for sequence analysis. This book describes the techniques for computer analysis of sequence data, with the emphasis on general issues rather than specific algorithms. Unlike many books on these topics, which focus on the "how-to" aspects of software packages, this one places more
emphasis on the science behind the packages and on interpretation of the results.
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Capillary Zone Electrophoresis
F. Foret ,
L. Krivankova , and
P. Bocek
Manufacturer: John Wiley & Sons Ltd (Import)
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ASIN: 3527300198 |
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Methods in zone electrophoresis
John R Sargent
Manufacturer: British Drug House Chemicals Ltd
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ASIN: 0950043915 |
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Mn is of toxicological concern because overexposure can lead to progressive, permanent neurodegenerative damage. Monomethyl-Mn-pentadienyl-tricarbonyl (MMT) is used as an anti-knock agent in fuel. Exhausted Mn compounds are absorbed in the lung and transported to the liver. Extended exposure causes an overflow of the liver with Mn species moving e.g. to the brain, causing irreversible central nervous system (CNS) disorders like Manganism. This paper focuses on experiments for getting more information on Mn species in liver extracts. The investigations are performed with respect to (1) a size characterization and (2) a subsequent identification of the Mn species in liver extracts using preparative size exclusion chromatography (SEC) followed by capillary zone electrophoresis coupled to inductively coupled plasma mass spectrometry (CZE-ICP-MS). First, extracts were analyzed using a mass calibrated SEC column coupled to ICP-MS detection. The chromatogram showed the ^5^5Mn-trace and proved main Mn elution between ca. 60-150kDa. Second, liver extracts were fractionated on the same SEC column, however, now the effluent was directed to a fraction collector. This resulted in fractions containing pre-purified, size characterized Mn species per fraction. It turned out that the Mn concentrations per fraction reflected roughly the previous on-line Mn trace. Third, the fractions were subject to CZE-ICP-MS, where the MS was operated additionally with dynamic reaction cell (DRC) technique. From size characterization (with SEC coupled on-line to ICP-MS or connected to a fraction collector and subsequent Mn determination in fractions) it was shown that most Mn species from liver extract were of high molecular mass (HMM) nature as they eluted mostly between 50 and 80min, corresponding to ca. 60-150kDa. With the two-dimensional speciation approach employing first SEC and then CZE-ICP-DRC-MS together with standard addition method, a series of Mn species was identified. Mn species predominantly were Mn-enzymes e.g. arginase, isocitric dehydrogenase, galactosyltransferase, prolidase, pyruvate carboxylase and oxalate oxidase. A typical Mn-transporter - Mn-albumin - was also seen, whilst Mn-transferrin obviously was degraded during SEC separation. This Mn-compound (independent whether as a standard or from liver extract) was not stable during SEC even at the finally chosen physiological conditions.
Book Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Mn is of toxicological concern because overexposure can lead to progressive, permanent neurodegenerative damage. Monomethyl-Mn-pentadienyl-tricarbonyl (MMT) is used as an anti-knock agent in fuel. Exhausted Mn compounds are absorbed in the lung and transported to the liver. Extended exposure causes an overflow of the liver with Mn species moving e.g. to the brain, causing irreversible central nervous system (CNS) disorders like Manganism. This paper focuses on experiments for getting more information on Mn species in liver extracts. The investigations are performed with respect to (1) a size characterization and (2) a subsequent identification of the Mn species in liver extracts using preparative size exclusion chromatography (SEC) followed by capillary zone electrophoresis coupled to inductively coupled plasma mass spectrometry (CZE-ICP-MS). First, extracts were analyzed using a mass calibrated SEC column coupled to ICP-MS detection. The chromatogram showed the ^5^5Mn-trace and proved main Mn elution between ca. 60-150kDa. Second, liver extracts were fractionated on the same SEC column, however, now the effluent was directed to a fraction collector. This resulted in fractions containing pre-purified, size characterized Mn species per fraction. It turned out that the Mn concentrations per fraction reflected roughly the previous on-line Mn trace. Third, the fractions were subject to CZE-ICP-MS, where the MS was operated additionally with dynamic reaction cell (DRC) technique. From size characterization (with SEC coupled on-line to ICP-MS or connected to a fraction collector and subsequent Mn determination in fractions) it was shown that most Mn species from liver extract were of high molecular mass (HMM) nature as they eluted mostly between 50 and 80min, corresponding to ca. 60-150kDa. With the two-dimensional speciation approach employing first SEC and then CZE-ICP-DRC-MS together with standard addition method, a series of Mn species was identified. Mn species predominantly were Mn-enzymes e.g. arginase, isocitric dehydrogenase, galactosyltransferase, prolidase, pyruvate carboxylase and oxalate oxidase. A typical Mn-transporter - Mn-albumin - was also seen, whilst Mn-transferrin obviously was degraded during SEC separation. This Mn-compound (independent whether as a standard or from liver extract) was not stable during SEC even at the finally chosen physiological conditions.
Book Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
In this work we report on the successful use of a composite prepared by dispersion of multi-wall carbon nanotubes (1-5@mm length, 20-50nm diameter) and copper microparticles within mineral oil as detector for amino acids quantification in flow injection analysis and capillary electrophoresis. The resulting electrode displays a highly sensitive amperometric detection of amino acids, based on the copper dissolution facilitated by the strong activity of amino acids as ligands of Cu(II). The sensor makes possible the detection of amino acids, electroactive or not, at very low potentials (0.000V) and physiological pH. A correlation between the sensitivity for the amino acids and the amount of copper within the composite is observed, demonstrating the importance of the metal in the sensor response. The best analytical performance is obtained for the electrode containing 12.0% (w/w) copper. The excellent results obtained with the carbon nanotube paste electrodes containing copper (CNTPE-Cu) as detector in flow systems makes them an interesting alternative for further analytical applications involving different bioanalytes.
Book Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Capillary zone electrophoresis separation with laser-induced fluorescence (LIF) detection for the determination of fluorescein isothiocyanate (FITC) derivatized stimulants (ephedrine, phenylpropanolamine and heptaminol) was demonstrated. A simple electrophoretic buffer system composed of 30mM triethylamine was selected and high voltage of 16kV was applied for the capillary electrophoresis separations. Stimulants in human urine were extracted by organic solvents and then derivatized with FITC. By employing a semiconductor laser-induced fluorescence detector, low concentration stimulants of 22ng/mL can be detected within 10min. Operation conditions for derivatization and separation were discussed. This simple CZE-LIF method with sensitive detection provided the possibility to develop the on-chip stimulants screening methods for doping control.
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- Absorbing collection on cryogenic engineering
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Advances in Cryogenic Engineering: Proceedings of the Cryogenic Engineering Conference - CEC Volume 47, Madison, Wisconsin, USA, 16-20 July 2001 (AIP Conference Proceedings)
Manufacturer: American Institute of Physics
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Binding: Hardcover
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ASIN: 0735400598 |
Book Description
All papers have been peer reviewed. The Cryogenic Engineering Conference covers applications and systems at temperatures where ordinary gases are liquids or solids. It covers the newest approaches to producing low temperatures and to the use of systems at low temperature such as new superconducting magnets, space applications and the properties at these temperatures. The design, construction, testing, and characterization of cryogenic systems are presented.
Customer Reviews:
Absorbing collection on cryogenic engineering.......2004-06-22
Comprehensive tome encompassing the proceedings of the 2001 Cryogenic Engineering Conference.
If you've ever wondered about extremely-low-temperature engineering, this is the one book to read. How to keep instrumentation on interplanetary spacecraft and earth-orbit satellites cold? It's here! Highly absorbing.
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Advances in Mechanics and Mathematics: Volume II (Advances in Mechanics and Mathematics)
Manufacturer: Springer
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ASIN: 1402076452 |
Book Description
Mechanics and mathematics have been complementary partners since Newton's time and the history of science shows much evidence of the beneficial influence of these disciplines on each other. Driven by increasingly elaborate modern technological applications the symbiotic relationship between mathematics and mechanics is continually growing. However, the increasingly large number of specialist journals has generated a duality gap between the two partners, and this gap is growing wider. Advances in Mechanics and Mathematics (AMMA) is intended to bridge this gap. Audience: This book is suitable for scientists, engineers and mathematicians, including advanced students (doctoral and post-doctoral level) at universities and in industry interested in modern mechanics and applied mathematics.
Book Description
Written between 1920 and 1937, when F. Scott Fitzgerald was at the height of his creative powers, these ten lyric tales represent some of the author's finest fiction. In them, Fitzgerald creates vivid, timeless characters -- a dissatisfied southern belle seeking adventure in the north; the tragic hero of the title story who lost more than money in the stock market; giddy and dissipated young men and women of the interwar period. From the lazy town of Tarleton, Georgia, to the glittering cosmopolitan centers of New York and Paris, Fitzgerald brings the society of the "Lost Generation" to life in these masterfully crafted gems, showcasing the many gifts of one of our most popular writers.
Customer Reviews:
An Out -of- Style Writer, Getting Down To Business.......2007-01-07
The literary voice of the ninteen-twenties' "Jazz Age," F. Scott Fitzgerald was out of step with the grimmer thirties. Facing his wife's insanity, increasing alcoholism, and his own obsolesence as a writer, the stories collected here show Fitzgerald facing his demons in bracingly honest prose. If "Crazy Sunday" and the other tales of the adventures of Pat Hobby, down-and-out screenwriter, feel a bit like autobiographical wallow, and "Family In The Wind," about a doctor in the midst of a country tornado, is an interesting if uncharacteristic journey into Steinbeck country, it's the title story of the collection that's worth the price of admission.
Charlie Wales is an ex-broker, returned to Paris after all the good times have gone, with only the goal of regaining custody of his daughter after the death of his wife. A thinly veiled take on Fitzgerald's own troubled relations with daughter Scottie after wife Zelda's madness, it's at once a suspenseful, moving, and lyrical story. All his powers are at work here, as if he knew this was his last shot at literary immortality, and he was just about right.
Hope, Illusion and Reality.......2005-12-31
F. Scott Fitzgerald is one of our greatest writers. He is best known today for his many wonderful novels, especially The Great Gatsby. As time has passed, his marvelous magazine stories have faded from sight . . . even though those were more widely read than his novels when they were written.
In Babylon Revisited: And Other Stories you will deepen your understanding of the novels . . . and of their author in these often semi-autobiographical tales. The best stories have as much impact as any of the novels in a spare exposition that adds to their power.
Each story deals with the same general theme: We live on hope which is based on illusions about reality. When faced with reality, we happily escape into new hopes based on different illusions. We are sort of like Peter Pan: We don't want to grow up.
The theme comes across with startling persuasiveness as Fitzgerald unpeels the many forms of hopeful illusions that will seem familiar to every reader.
The stories build chronologically across the backdrop of the United States after World War I in the 20's and 30's. That shift in authorship times also inadvertently adds the drama of seeing how the psychology of the young and educated changed as American went from mindless boom to seemingly unending bust.
Fitzgerald has a rich imagination to makes his world open up for readers so that you can feel both the physical sensations and the emotions of the characters . . . and become the characters while you are reading.
The stories themselves have that delightful quality of exaggeration that makes his points indelible.
The Ice Palace explores a Southern beauty's pursuit of an advantageous marriage in the frozen tundra of Minnesota in winter. May Day recounts the pursuit of pleasure and accomplishment by those of various social classes and beliefs. The Diamond as Big as the Ritz is a wild tale of a mythical place and the consequences of unlimited wealth. Winter Dreams deals with the painful consequences of acting on the illusions of romantic love. Absolution is an amazing story about how we can carelessly end up being untrue to God and ourselves. The Rich Boy considers how being rich and powerful can get in the way of being close to others. The Freshest Boy looks at being an awkward teenage boy and how he came to make peace with the world. Babylon Revisited shows how our mistakes can come home to roost after we believe we are invulnerable. Crazy Sunday is an astonishing look at the psychology of how we connect to one another through others. The Long Way Out is about a woman who suffers from a mental collapse and is now ready to return to her husband . . . when fate steps in.
My favorite stories in the book are May Day, The Diamond as Big as the Ritz, The Freshest Boy, Babylon Revisited and Crazy Sunday.
If you haven't read these stories before, you have a great treat ahead of you. If you can find a copy of George Guidall's narration for Recorded Books, your pleasure will be even greater.
Babylon Revisited is Timeless and Apt.......2005-12-01
The Book of Revelations in the New Testament is the most likely source from which F. Scott Fitzgerald draws his "Babylon Revisited". In Revelations, Babylon the Great (also an ancient Near Eastern city of materialism and sexual excess) is the `mother of whores' and the source of all evil in the Roman Empire. She is said to have been defeated by God and judged for her excessive sin. Upon her destruction, the saints rejoice while the merchants and hedonistic pleasure seekers morn. Symbolism abounds in this revision of the timeless tale and the choice of Fitzgerald's title could not be more appropriate.
Charlie himself is the regeneration of Babylon. During the economic boom of the 20's, Charlie and his wife lived life to its fullest and most shallow degree. They partied until sunup. They squandered wealth. We even get the impression that there was a significant amount of infidelity existing on both sides. As with Babylon, Charlie is punished: The stock market crash in 1929 liberates him of a fortune, "his child [is] taken from his control, [and] his wife escaped to a grave in Vermont."
As with Babylon, Charlie's fall had its rejoicers and mourners. Marion, his wife's bereaved sister, saw Charlie's fall as an opportunity to gain control of his child, and with sincere intentions rid her family of the sinner. Though she doesn't expressly rejoice in her brother-in-laws demise, she does blame him for her sister's death and understands why his life has turned out askew. Duncan and Lorraine, on the other hand, mourned the loss of their sinister partner in indulgence.
This story is complete with all of the historic reference and symbolism that has come to define F. Scott Fitzgerald. What a fantastic, unbelievably creative writer. It's amazing how timeless his writings are, and "Babylon Revisited" is the perfect example of that fact. It really makes you think about your own life.
Genius As Big As The Ritz.......2005-01-28
The king of the 1920's Lit World wrote short stories for big money in Scribner's Magazine, Collier's, Esquire, and Saturday Evening Post. His first novel made him famous, This Side of Paradise, but his subsequent novels including The Great Gatsby sold meagerly. Zelda and Scott went through dough like drunken sailors, so Scott wrote short stories for a quick buck. This group of stories is among his best and though some or all were written commercially, Scott's talent was so huge that they rival his chief competitor's: Hemingway, Parker, Anderson, and Larder in charm and precision.
Above all, Fitzgerald is charming. The drunken rich boys of May Day are close to the authors experience and poignantly revealing. Scott was the son of a failed businessman. His mother's family was well to do and Scott associated with rich beauties that seemed always just beyond a snow covered golf course as in Winter Dreams. His experience with his future wife, Zelda Sear, an Alabama debutante is cloaked in fantasy in Ice Palace. Surely newlyweds are surprised to find they have married strangers. In that there is no secret, but Fitzgerald gives his bride a hysterical nightmare in a St Paul carnival ice maze. The reader loves Sally Carrol and is genuinely caught up in her dilemma of Minnesota in-laws and a suddenly stern husband.
Fitzgerald was a dreamer and The Diamond As Big As the Ritz is a parable about a family so rich, and so self-centered in their luxuries, they murder their guests less the secret of the their wealth be known. In an era where a million dollars could buy a country, Fitzgerald's fascination with success and the rich permeates his work.
BRILLIANT STORIES.......2000-12-27
I bought this volume of stories simply to get a copy of Fitzgerald's "May Day" which I'd read in one of my college texts and then could not find for years. I have always felt that "May Day" would make a superb film--and the screenwriter could lift most of the dialogue right out of the story. It is that good and simple and dramatic. Actually every one of the stories in this collection is first rate. Here is Fitzgerald, only in his 20's, writing of American aspirations before, during and after World War I. And no one wrote about this subject better than he did. The characters are rich and complex, all of them dissatisfied with the bones that life has thrown them, all of them desiring what others have. The reader sees their foibles and loves them anyway. These are not perfect people. They are real people in a time of trouble--fighting, most of them, simply to stay afloat in a world changing faster than anyone would have thought possible. I cannot recommend these brilliant stories highly enough. There is also a brief life and appreciation of Fitzgerald in this lovely Scribner edition.
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Babylon Revisited & Other Stories
F Scott Fitzgerald
Manufacturer: CHARLES SCRIBNERS SONS LTD
ProductGroup: Book
Binding: Paperback
Fitzgerald, F. Scott
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ASIN: B000TXCJIY |
Product Description
Unabridged: Babylon Revisited, The Lost Decade, The Bridal Party, Three Hours Between Planes.
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BABYLON REVISITED AND OTHER STORIES
F. Scott Fitzgerald
Manufacturer: Charles Scribner's Sons
ProductGroup: Book
Binding: Paperback
Fitzgerald, F. Scott
| Classics
| United States
| World Literature
| Literature & Fiction
| Subjects
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ASIN: B000OY8DQK |
Average customer rating:
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Babylon Revisited and Other Stories
F. Scott Fitzgerald
Manufacturer: Charles Scribner's Sons
ProductGroup: Book
Binding: Paperback
Fitzgerald, F. Scott
| Classics
| United States
| World Literature
| Literature & Fiction
| Subjects
| Books
ASIN: B000L2LX58 |
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babylon revisited and other stories
Manufacturer: scribner
ProductGroup: Book
Binding: Paperback
Fitzgerald, F. Scott
| Classics
| United States
| World Literature
| Literature & Fiction
| Subjects
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ASIN: B000HBQZKQ |
Average customer rating:
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BABYLON REVISITED AND OTHER STORIES
Manufacturer: Charles Scribner
ProductGroup: Book
Binding: Paperback
Fitzgerald, F. Scott
| Classics
| United States
| World Literature
| Literature & Fiction
| Subjects
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ASIN: B000HFFZEO |
Average customer rating:
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BABYLON REVISITED AND OTHER STORIES
F. SCOTT FITZGERALD
Manufacturer: Charles Scribner's Sons
ProductGroup: Book
Binding: Hardcover
Fitzgerald, F. Scott
| Classics
| United States
| World Literature
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ASIN: B000K0H27Y |
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