Essential Cell Biology: A Practical Approach Volume 1: Cell Structure (Practical Approach Series, 262, etc.)

Book Description

Biological and medical research relies upon an integrated understanding of the molecules within cells and of the interactions between cells. This has imposed great demands on investigators. Being an expert in a relatively narrow area is no longer sufficient as many studies now require the
use of a wide range of techniques to provide the necessary integration. A lack of familiarity with the experimental possibilities can make such diversification difficult to achieve. This two volume set of Essential Cell Biology is designed to help researchers overcome these problems. It has not
been possible to include all of the techniques available in cell biology so the challenge was to identify those that might be most relevant to researchers who are new to this topic. We have tried to cover both traditional and more recent approaches. The theory and basic principles of each technique
are described, together with detailed protocols and advice for trouble shooting. Directions to more specialised techniques are also included. We hope the result inspires readers to experience the challenges and rewards of cell biology research for themselves and to contribute to the ongoing task
of understanding the life of the cell.

Essential Cell Biology volume 1 focuses on techniques for studying cell structure whilst volume 2 concentrates on understanding how the cell functions. Volume 1 details the essential background information and protocols for observing and understanding cell morphology and cell structure, including,
for example, investigations of nucleic acids, lipids, and the cytoskeleton. This is the essential guide to cell biology for researchers new to the field.

Platelets and Megakaryocytes: Volume 1: Functional Assays (Methods in Molecular Biology)

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Platelets and Megakaryocytes: Volume 1: Functional Assays (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588291014

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For both volumes:

A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods for the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Cardiovascular Disease, Volume 1: Genetics (Methods in Molecular Medicine)

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Cardiovascular Disease, Volume 1: Genetics (Methods in Molecular Medicine)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

Genetics | Basic Science | Medicine | Subjects | Books

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ASIN: 1588295729

Book Description

The two volumes of Cardiovascular Disease: Methods and Protocols provide comprehensive coverage of both basic and advanced approaches to the study and characterization of cardiovascular disease. In Volume 1: Genetics and Volume 2: Molecular Medicine, highly experienced cardiovascular researchers describe in detail the most important techniques in molecular medicine that are employed in genetic, molecular, cellular, structural, and physiological studies of cardiovascular disease.
A total of 37 chapters cover cutting-edge methods including cytogenetic analyses, linkage programs for mapping chromosomal locations of disease genes, bioinformatics, as well as human genetics for identifying genes for both monogenic and common complex diseases. Other areas covered also include mouse genetics for identifying genes for complex disease traits, microarray (genechips) analysis, proteomics, cell biology, physiology, animal models of human disease, gene therapy, vascular biology, and stem cells.
The theory and principles of each method are described in detail, followed by a thorough description of materials and equipment needed, and step-by-step protocols for successful execution of the method. A Notes section provides advice for potential problems, any modifications, and alternative methods.
Comprehensive and timely, both volumes of Cardiovascular Disease: Methods and Protocols serve as a valuable resource book for active researchers looking to advance knowledge of the mechanisms, diagnoses, and treatments of cardiovascular disease.

Checkpoint Controls and Cancer: Volume 1: Reviews and Model Systems (Methods in Molecular Biology)

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Checkpoint Controls and Cancer: Volume 1: Reviews and Model Systems (Methods in Molecular Biology)

Manufacturer: Humana Press
ProductGroup: Book
Binding: Hardcover

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ASIN: 1588292142

Book Description

Prestigious researchers working at the cutting-edges of their fields comprehensively review the complexities of checkpoint controls and the model systems available to study them. The authors introduce all of the important components of checkpoint controls, describe their intricate interactions, and highlight the relevance of these processes to the cancer problem. Additional chapters illustrate the advantages of using such diverse model systems as intact human skin, knockout mice, Xenopus, and yeast, and show how they can cross-fertilize and accelerate research both across disciplines and beyond the boundaries of a particular species. A second volume, Activation and Regulation Protocols, provides readily reproducible experimental protocols to study the molecular components of checkpoint controls and their regulation.

Cell Biology, Volume 1, Second Edition: A Laboratory Handbook (Cell Biology)

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Greatest value of this work is its comprehensiveness

Cell Biology, Volume 1, Second Edition: A Laboratory Handbook (Cell Biology)

Manufacturer: Academic Press
ProductGroup: Book
Binding: Paperback

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Molecular Biology of the Cell, Fourth Edition

ASIN: 0121647269

Book Description

This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together new and revised chapters. Each chapter is concisely written and beautifully illustrated, making the attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors.

Key Features
* Contains more than 40% new material
* Provides cell biologists and other life scientists with the most up-to-date instructions for basic and advanced cell biological techniques, including those at the interface between cell and molecular biology
* Features uniform style and editing and includes contributions from world-renowned authorities in their respective fields
* Contains information appropriate for a large, diverse, and constantly growing international audience of cell, developmental, and molecular biologists, plus others who need these methods in their laboratory research
* Includes color plates throughout the set for easy reference
* Designed as the essential lab guide and research reference for the field

Customer Reviews:

Greatest value of this work is its comprehensiveness.......2001-02-03

Cell biology techniques have been continuously upgraded since the middle of this century, and future developments seem assured. In the 1990s, alone, new developments have led to improved separations capability, detection instrumentation, and new formulations of serum-free media. Any research institution with faculty and students engaged in cell biology will want to have the most current laboratory guidelines available, and this four-volume, peer-reviewed collection of protocols answers that need. The second edition has benefited from suggestions from practicing scientists, filling in gaps from the first edition.

Perhaps the greatest value of this work is its comprehensiveness. Laboratories and libraries can have detailed guidelines for cell culture, separation, staining, growth, apoptosis, preparation of component parts, assays, antibody production, genetic engineering, microscopy, electrophoresis, and more, all in one source. Need to know how to produce transgenic mice? It's here. How to grow viruses? Prepare nuclei? Stain mitochondria? Use fluorecence microscopy? All here.

Book Description

The major histocompatibility complex is the subject of much research in the immunology field. There is a great deal of interest in the structure of MHC proteins and in their function as antigen presenting molecules. An increasing number of immunology laboratories are preparing to investigate biochemical and genetic techniques in order to study these molecules. The identification of the peptide transporter genes and the elution of peptides from MHC molecules demonstrate how rapidly our understanding of the structure and function of the MHC locus has advanced. MCH1: A Practical Approach brings together the technologies which facilitate the advances that are being made. It has detailed explanations of processes such as peptide translocation into the ER, the application of mass spectrometry to the analysis of peptides bound to MHC, and signal detection via MHC class II molecules which can lead to either activation or programmed cell death. This book is a comprehensive and thorough guide to the fast growing and fascinating field of MHC research. Graduates, post doctorial researchers, academic staff and clinicians in the immunology field will find this book an invaluable aid.

Advanced Placement Biology Laboratory Manual for Students Exercises 1-12 Edition D

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Advanced Placement Biology Laboratory Manual for Students Exercises 1-12 Edition D
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Manufacturer: The College Board
ProductGroup: Book
Binding: Paperback
ASIN: B000WE1HWO

ADVANCED PLACEMENT BIOLOGY TEACHER'S LABORATORY PREPARATION MANUAL Exercises 1-12

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ADVANCED PLACEMENT BIOLOGY TEACHER'S LABORATORY PREPARATION MANUAL Exercises 1-12

ProductGroup: Book
Binding: Paperback
ASIN: B000TRMGWE

Product Description

To accompany AP biology laboratory manual for students - Edition C

Advanced Placement: Biology (Laboratory manual for students Exercises 1-12)

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Advanced Placement: Biology (Laboratory manual for students Exercises 1-12)

Manufacturer: College Entrance Examination Board
ProductGroup: Book
Binding: Paperback
ASIN: B000VWXIVU

Product Description

College biology Laboratory regulation.

Antibodies: A Practical Approach Volume 1 (Practical Approach Series)

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Antibodies: A Practical Approach Volume 1 (Practical Approach Series)

Manufacturer: Oxford University Press, USA
ProductGroup: Book
Binding: Hardcover

Immunology | Basic Science | Medicine | Subjects | Books

Book Description

Antibodies have become the preferred, and often essential, tools to identify, quantify and probe the structure and biological properties of an enormous range of antigenic molecules. This volume describes the properties of antibodies and antigens and provides detailed protocols for the
production and quality control of polyclonal, murine monoclonal and human monoclonal antibodies. Step-by-step descriptions are given for antibody methods. In addition to giving precise methodological details, the contributors consider the applications and appropriate use of these
techniques.

Book Description

As exciting as the new field of genomics is, it has not yet produced a basic conceptual change in biology. The fundamental problems remain: the origin of life, cell organization, the pathways of differentiation, aging, and the molecular and cellular capabilities of the brain. What has occurred is an explosion of molecular information obtained by genomic sequences, which will soon be followed by exhaustive catalogs of protein interactions and protein function. This wealth of information can be analyzed and manipulated only with the help of computers. The rapidly expanding role of computers in biology may usher in a profound conceptual change in how we study living systems in the laboratory.

This book focuses on current computational approaches to understanding the complex networks of metabolic and gene regulatory capabilities of the cell. The contributors look well beyond the state of the art in computational biology to anticipate what biological research will be like in a post-genomic world.

Customer Reviews:

Good overview and literature survey of modern developments.......2006-12-10

The determination of the genome of organisms is quite an achievement, and one that is taking place with incredible rapidity at the present time. But in order to completely understand the life processes of an organism one needs to understand how the genes are expressed and the consequent effects on the metabolism. As is pointed out in this book, even for the bacterial organism Escherichia coli, whose genome is the most understood in the biosphere, only about six hundred promoters have been determined experimentally. If genome determination is to have practical applications to human and animal health, it will become necessary to have a much better understanding of how the metabolism of an organism is regulated by its genes. This is an enormously difficult undertaking due to the magnitude of the information involved. It is therefore no surprise that computing machines and algorithms have been used to sort through and organize this information. Whether it goes by the name of computational biology or computational genomics, the use of these machines has assisted greatly in the understanding of the life processes. This book outlines a few of the approaches that have been taken. It is directed towards experts, but it could be read by anyone who has an interest in genetic and metabolic engineering from an information-theoretic, computational perspective. The book was first published four years ago, and so it is somewhat out of date, due to the increases in CPU processing speeds and new algorithms that have been developed since then.

Of particular interest is the article by Collado-Vides et al and which concerns the use of software to translate a DNA sequence into all possible proteins so as to compare them against a database of proteins that were determined by experiment. The authors discuss how this can be done for the case of E. coli and for a database called RegulonDB, which can be accessed on the Web, and which contains information on transcription initiation and operon organization in the K-12 strain of E. coli. The goal of the authors is to understand the structure of gene regulatory networks, both from a purely computational point of view and using experimentally determined transcription profiles. The article is broken down into four parts, with the first one concentrating on the prediction of gene regulatory networks using sequence information, followed by one discussing the use of Bayesian networks to assist in these predictions. The third part gives examples on how to use the database information to analyze transcription experiments and the last puts all of this analysis in the context of evolutionary origins of transcriptional regulation.

The authors are very interested in the process of transcription, particularly the initiation phase. As is well known, gene transcription is catalyzed by the RNA polymerase (RNAP) proteins. In particular, the RNAP `holoenzyme' contains the `sigma factor' that guides RNAP to specific sequences on the genomic DNA (the promoter). The RNAP holoenzyme with the sigma factor thus recognizes the precise site where transcription is initiated. Having located a promoter and binding to it, the RNAP holoenzyme and the bound DNA then undergo a series of conformational changes that allow RNA synthesis to be initiated. For the case of E. coli, the study of the formation of RNAP contacts at these specific sites on the promoter DNA has been the subject of intense experimental investigation, the challenge being the measurement of the formation of these contacts in real time. In this context the author's goal is find computational schemes that will predict the promoters and other protein-binding sites. This has traditionally been done with the use of weight matrices, but as the authors point out this method (and others) results in a large number of false positives. To improve on this method, they first obtain a population of potential candidates using a low enough threshold that weaker sites can be identified. The next step is to select a collection of promoters by comparing them with all candidates within the regulatory region. The candidates are split into groups separated by the distance to the beginning of the gene, and each group contributes one best candidate. This method, the authors claim, leads to one true promoter out of six candidates on the average.

The authors though do not end the analysis here, as they believe that the prediction of promoters is not reliable enough to search for sites for operator sites for specific transcriptional regulators (the information content of the promoter sites is too low). They therefore make use of a `dyad-detector' algorithm that has the ability to identify oligonucleotides up to seven bases long that are overrepresented in a given family of upstream regions. The dyad-detector can also find words with internal symmetry, such as palindromes. Also of interest to the authors is the identification of transcriptional activators and repressors that affect transcription initiation. Their goal is to identify which protein binds to which set of sites, assuming that a collection of protein-binding motifs has been identified and that the number in this collection is equal to the number of transcriptional regulators.

Book Description

A classic study of the beliefs and institutions of mankind, and the progress through magic and religion to scientific thought, The Golden Bough has a unique status in modern anthropology and literature. First published in 1890, The Golden Bough was eventually issued in a twelve-volume edition (1906-15) which was abridged in 1922 by the author and his wife. That abridgement has never been reconsidered for a modern audience. In it some of the more controversial passages were dropped, including Frazer's daring speculations on the Crucifixion of Christ. For the first time this one-volume edition restores Frazer's bolder theories and sets them within the framework of a valuable introduction and notes. A seminal work of modern anthropolgy, The Golden Bough also influenced many twentieth-century writers, including D H Lawrence, T S Eliot, and Wyndham Lewis. Its discussion of magical types, the sacrificial killing of kings, the dying god, and the scapegoat is given fresh pertinence in this new edition.

Customer Reviews:

Golden Bough.......2007-09-26

A approach of academic study to common reader interest. A excellent book for ritual understanding. The Frazer's Golden Bough is one of more sold around the world and is also one of more controversial within academic society because its author broken one of its dogmas - written without the academic language vicious.

An influential work on four 20th century seminal works.......2007-09-23

This book is a seminal work because it had a crucial influence on four important works of the twentieth century: T. S. Elliott's poem the Waste Land, Joseph Campbell's Hero with a Thousand Faces, Dan Brown's book The Da Vinci Code, and Francis Ford Coppolla's movie Apocalypse Now, screenplay by John Milius.

Sir James George Frazer's book written in 1922 was a groundbreaking work on ancient religion, paganism, and roots of early Christianity. Frazer does an in-depth examination of the sacrificial killing of god-kings to ensure bountiful harvests, which Frazer traces through several cultures, including in his elaborations the myths of Adonis, Osiris, and Balder.

Frazer spent his life writing fifteen volumes of history of myth and religion. This book sums up his theory of magic and its connections to paganism, as well as fusing ideas from Jessie L. Weston's book From Ritual To Romance and Gnostic texts that serve as a link to early Christianity's influence from ancient nature cults. His chapter titles say much about where his work goes and why it is so influential on iconic twentieth century works. The King of the Wood explains the original nature of the task imposed upon the hero, it undoubtedly influenced both Campbell's and Coppola's works. The Myths of Adonis, Attis, and Osiris looks to establish a chain of descent connecting early Aryan and Babylonian ritual with classic, Medieval and modern forms of nature worship. Our Debt to the Savage explains the role of the Medicine Man or doctor in fertility ritual. The Killing of the Devine King analyzes how this title is prevalent in so many of humankind's legends, and was a definite influence on Coppola's Colonel Kurtz character. Sacrifice of the King's Son regarded as an object of awe certainly influenced The Da Vinci Code.

Frazer's book is interesting and fun to read. I especially became interested in it from the movie Apocalypse Now. There is a scene in the movie that shows Colonel Kurtz's nightstand in his cave. Weston's book is one of three on the nightstand. The other two are Joseph Conrad's Heart of Darkness, which the film is based on. The other book is Jessie L. Weston's book From Ritual To Romance. Anyone wanting to understand the movie Apocalypse Now, especially the character of Colonel Kurtz, and what Milius and Copolla were trying to tell their audience need to read these three books!

As a graduate student reading in philosophy and history I recommend this book for anyone interested in literature, myth, history, philosophy, religion and fans of Apocalypse Now.

The real deal.......2007-07-04

I'll skip reviewing the content and speak to book's edition. This is the one that was abridged by the author from a multi-volume, earlier edition. In later years, the tome was watered down and censored due to authorial speculation on the nature of Jesus. All the controversial ideas are present in this particular edition, so it is safe to purchase it and not feel cheated.

Finally!.......2007-01-03

An "uncensored" abridged version,
full of speculation about Christ. Sure to get the evangelicals lighting their torches,
to storm this Castle of Prometheus.

The Golden Bough by Sir James George Frazer.......2006-06-29

This is a wonderful book on the origin of beliefs, culture and
classic ceremonies. For instance, the Native American Indians
regarded a person's name as a part of their personality.
In Bohemia, children carry a straw man out of the village to
cast out death. Aphrodite and Old Paphos constitute one of the
most celebrated shrines in the ancient world. In death and
resurrection, Egyptians celebrated life after death. At Lagos in
Guinea, young women were impaled by custom after spring equinox
in order to secure a good crop that year. Festivities were
prepared in order to coincide with the summer and winter solstices.

The work would be perfect for students of world culture,
fine arts, language and literature.

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